Human Pepsinogen A Antibody

Catalog #: MAB7525 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7525
MAB7525-SP

Detection of Human Pepsinogen A by Western Blot.

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All Pepsinogen A Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Pepsinogen A Antibody Summary

Species Reactivity

Human

Specificity

Detects human Pepsinogen A4/PGA4 in direct ELISAs. Human PGA3 and PGA5 reactivity has not been demonstrated but is likely due to their high homology with human PGA4. In direct ELISAs, no cross-reactivity with recombinant human (rh) Pepsinogen C, rhCathepsin D, or rhCathepsin E is observed.

Source

Monoclonal Mouse IgG₁ Clone # 762015

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Pepsinogen A4/PGA4
Ile16-Ala388
Accession # P00790

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Pepsinogen A by Western Blot. Western blot shows lysates of human stomach tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Pepsinogen A Monoclonal Antibody (Catalog # MAB7525) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Pepsinogen A at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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Pepsinogen A in Human Stomach. Pepsinogen A was detected in immersion fixed paraffin-embedded sections of human stomach using Mouse Anti-Human Pepsinogen A Monoclonal Antibody (Catalog # MAB7525) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to epithelial cells in gastric glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pepsinogen A

Pepsins are aspartic proteases that are synthesized in the gastric mucosa and secreted into the stomach. They are released as zymogens called pepsinogens and then converted to active pepsins by the acidic pH of gastric juices (1). PGA3, PGA4, and PGA5 are human Pepsinogen A isozymogens that differ in sequence by 2‑4 amino acids (aa). Human pepsinogen A isozymogens share approximately 56% amino acid sequence identity with mouse and rat pepsinogen A isozymogens. Pepsins have optimal activity under conditions of acidic pH and are inhibited by pepstatin. Pepsin A has broad substrate specificity, but preferentially cleaves peptide bonds involving aromatic and aliphatic amino acids.

Entrez Gene IDs

643847 (Human)

Alternate Names

EC 3.4.23; EC 3.4.23.1; FLJ58952; FLJ77962; pepsin A; pepsinogen 4, group I (pepsinogen A); Pepsinogen A; pepsinogen A4; PGA3; PGA5