Summary
Precision
Recovery
Linearity
Data Examples
Product Datasheets
Preparation and Storage
Related Research Areas
Human Perforin QuicKit ELISA Summary
Assay Length
80 minutes
Specificity
Natural and recombinant human Perforin.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine QuicKit Human Perforin Immunoassay is a one step, 80-minute solid phase ELISA designed to measure human Perforin levels in cell culture supernates, serum, plasma, and cell lysates. It contains recombinant human Perforin and antibodies raised against the recombinant protein. Results obtained for naturally occurring human Perforin showed linear curves that were parallel to the standard curves obtained using the recombinant QuicKit standards. These results indicate that this kit can be used to determine relative mass values for natural Perforin.
Precision
Intra-Assay Precision (Precision within an assay) Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision. Assays were performed by at least five technicians
Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 20 | 20 | 10 | 10 |
| Mean (pg/mL) | 647 | 2885 | 477 | 2689 |
| Standard Deviation | 36.6 | 77.7 | 33 | 95.4 |
| CV% | 5.7 | 2.7 | 6.9 | 3.5 |
Recovery
The recovery of human Perforin spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 103 | 82-132 |
| EDTA Plasma (n=2) | 100 | 81-122 |
| Heparin Plasma (n=2) | 103 | 87-118 |
| Lysis Buffer (n=1) | 118 | 109-127 |
| Serum (n=2) | 97 | 73-117 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of human Perforin were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
Scientific Data
Product Datasheets
Preparation and Storage
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
FAQs
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