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Human Perilipin Antibody

Catalog #: AF6615
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF6615
AF6615-SP
Detection of Human Perilipin by Western Blot.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Perilipin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Perilipin in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Perilipin
Thr8-Ala145
Accession # O60240
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Perilipin antibody by Western Blot. View Larger

Detection of Human Perilipin by Western Blot. Western blot shows lysates of human heart (adipose) tissue. PVDF Membrane was probed with 0.25 µg/mL of Human Perilipin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6615) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Perilipin at approximately 55-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Perilipin

Perilipin (Perilipin-1/A; also PLIN1 and Lipid droplet-associated protein) is a 62 kDa constitutively phosphorylated protein that belongs to the PAT (Perilipin/Adipophilin/TIP47) family of molecules. Found primarily in mature adipocytes, perilipin-1 interacts with lipid-coat proteins at the edge of the fat droplet and blocks lipase activity. Upon phosphorylation, perilipin-1 becomes a 65-67 kDa molecule that likely promotes the dispersion of docking molecules and provides a scaffold for lipase at the lipid droplet surface. Human perilipin-1 is 522 amino acids (aa) in length. It contains a lipid droplet targeting region (aa 233-364) that contains a polyGlu segment (aa 307-316). Phosphorylation at the N-terminus is necessary for lipase interaction with lipid. One 50-54 kDa human splice variant (perilipin-B) is reported that shows an 85 aa substitution for aa 404-522. This apparently interacts with cell membrane triglycerides. Over aa 8-145, human perilipin-1/A shares 97% aa identity with mouse perilipin-1.

Entrez Gene IDs
5346 (Human); 103968 (Mouse); 25629 (Rat)
Alternate Names
PERI; perilipin 1; Perilipin; perilipin-1; PLIN; PLIN1; PLINLipid droplet-associated protein

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Citations for Human Perilipin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Mitotane Targets Lipid Droplets to Induce Lipolysis in Adrenocortical Carcinoma
    Authors: Kate M Warde, Yi Jan Lim, Eduardo Ribes Martinez, Felix Beuschlein, Paula O’Shea, Constanze Hantel et al.
    Endocrinology
  2. Distinct Bone Marrow Sources of Pleiotrophin Control Hematopoietic Stem Cell Maintenance and Regeneration
    Authors: HA Himburg, CM Termini, L Schlussel, J Kan, M Li, L Zhao, T Fang, JP Sasine, VY Chang, JP Chute
    Cell Stem Cell, 2018-08-09;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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