Human Periostin/OSF-2 Antibody

Catalog #: MAB35484 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB35484-100
MAB35484-SP

Human Periostin/OSF‑2 ELISA Standard Curve.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Periostin/OSF-2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Periostin/OSF-2 in direct ELISAs.

Source

Recombinant Monoclonal Mouse IgG_2A Clone # 613643R

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Periostin/OSF-2
Met1-Gln836
Accession # Q15063

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Periostin/OSF‑2 Monoclonal Antibody (Catalog # MAB35483).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Periostin/OSF-2 DuoSet ELISA Kit (Catalog # DY3548B) for convenient development of a sandwich ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human Periostin/OSF‑2 ELISA Standard Curve. Recombinant Human Periostin/OSF-2 protein was serially diluted 2-fold and captured by Mouse Anti-Human Periostin/OSF-2 Monoclonal Antibody (Catalog # MAB35483) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Periostin/OSF-2 Monoclonal Antibody (Catalog # MAB35484) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Periostin/OSF-2

Periostin, also known as OSF-2, is a secreted matricellular protein with functions in extracellular matrix formation, cell migration, and inflammation (1). It is secreted as a 90 kDa monomer that can aggregate into >170 kDa higher-order multimers (2). Periostin contains an N-terminal EMI domain followed by four tandem FAS1 domains [takeshita]. Mature human Periostin shares 91% aa sequence identity with mouse and rat Periostin. Alternative splicing generates additional isoforms with various deletions in the C-terminal region following the FAS domains. Periostin is expressed by mesenchymal cells such as vascular smooth muscle cells, fibroblasts, osteoblasts, and odontoblasts in developing teeth (4-7). It is up-regulated in many carcinomas (2, 8). Periostin binds to Integrins alpha _v beta ₃ and alpha _v beta ₅ (2, 9), leading to enhanced cell adhesion and cell migration (2, 5, 6). It enhances Fibronectin and Collagen I production and promotes collagen fibrillogenesis (10, 11). It also induces epithelial-mesenchymal transition, tumor growth, invasion, and metastasis (9). Periostin induces the expression of VEGF R2 on endothelial cells and VEGF-C in tumor cells, and it can induce tumor lymphangiogenesis (8, 12). Periostin plays an important role in heart valve development and tissue healing after myocardial infarction (5, 13, 14). In asthma, it is up-regulated in bronchial epithelium and plays both destructive and protective roles by inducing eosinophil infiltration and inhibiting goblet cell metaplasia and mucus production, respectively (15, 16).

References

Liu, A.Y. et al. (2014) Matrix Biol. 37:150.
Gillan, L. et al. (2002) Cancer Res. 62:5358.
Takeshita, S. et al. (1993) Biochem. J. 294:271.
Kruzynska-Frejtag, A. et al. (2004) Dev. Dyn. 229:857.
Lindner, V. et al. (2005) Arterioscler. Thromb. Vasc. Biol. 25:77.
Horiuchi, K. et al. (1999) J. Bone Miner. Res. 14:1239.
Li, G. et al. (2006) Atherosclerosis 188:292.
Shao, R. et al. (2004) Mol. Cell. Biol. 24:3992.
Yan, W. and R. Shao (2006) J. Biol. Chem. 281:19700.
Erkan, M. et al. (2007) Gastroenterology 132:1447.
Norris, R.A. et al. (2007) J. Cell. Biochem. 101:695.
Kudo, Y. et al. (2012) PLoS One 7:e44488.
Snider, P. et al. (2008) Circ. Res. 102:752.
Kuhn, B. et al. (2007) Nat. Med. 13:962.
Blanchard, C. et al. (2008) Mucosal Immunol. 1:289.
Sehra, S. et al. (2011) J. Immunol. 186:4959.

Long Name

Osteoblast Specific Factor 2

Entrez Gene IDs

10631 (Human); 50706 (Mouse); 361945 (Rat)

Alternate Names

Fasciclin I-like; MGC119510; MGC119511; OSF2; OSF-2; OSF-2osteoblast specific factor 2 (fasciclin I-like); OSF2periodontal ligament-specific periostin; Osteoblast-specific factor 2; PDLPOSTN; periostin isoform thy2; periostin isoform thy4; periostin isoform thy6; periostin isoform thy8; Periostin; periostin, osteoblast specific factor; PNRP11-412K4.1; POSTN; TRIF52