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Human Phospho-AMPK alpha 1/2 (T183/T172) Antibody

Catalog #: AF2509
2 Citations Datasheet / COA / SDS

Discontinued Product

AF2509 has been discontinued.
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Detection of Human Phospho-AMPK alpha 1/2 (T183/T172) by Western Blot.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Phospho-AMPK alpha 1/2 (T183/T172) Antibody Summary

Species Reactivity
Human
Specificity
Detects human AMPK alpha 1 and AMPK alpha 2 when phosphorylated at T183 and T172, respectively, in Western blots.
Source
Polyclonal Rabbit IgG
Purification
Antigen and protein A Affinity-purified
Immunogen
Phosphopeptide containing human AMPK alpha 1 T183 site
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Phospho-AMPKa1/2 (T183/T172) antibody by Western Blot. View Larger

Detection of Human Phospho-AMPK alpha 1/2 (T183/T172) by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line treated with 1 µM ionomycin for the indicated times in minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-AMPKa1/2 (T183/T172) Antigen Affinity-purified Polyclonal Antibody, followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-AMPKa1/2 (T183/T172) at approximately 63 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AMPK alpha 1/2

AMP-activated protein kinase (AMPK) is a heterotrimeric complex consisting of a catalytic alpha subunit and regulatory beta and δ subunits. The alpha subunit of AMPK is activated allosterically by AMP, and by phosphorylation via the AMPK kinase LKB1. Active AMPK down-regulates anabolic pathways such as fatty acid and cholesterol synthesis, and up-regulates catabolic pathways such as glycolysis and fatty acid oxidation.

Long Name
AMP-activated Protein Kinase alpha 1/2
Alternate Names
AMPK alpha 1/2; AMPK; AMPKa1; PRKAA1 protein kinase, AMP-activated, alpha 1 catalytic subunit

Product Datasheets

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Citations for Human Phospho-AMPK alpha 1/2 (T183/T172) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Metformin, an AMPK Activator, Inhibits Activation of FLSs but Promotes HAPLN1 Secretion
    Authors: Y Chen, F Qiu, B Yu, Y Chen, F Zuo, X Zhu, KS Nandakumar, C Xiao
    Mol Ther Methods Clin Dev, 2020-05-13;17(0):1202-1214.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Progesterone receptor membrane component 1/Sigma-2 receptor associates with MAP1LC3B and promotes autophagy.
    Authors: Mir, Shakeel, Schwarze, Steven R, Jin, Ling, Zhang, Jinling, Friend, Woodrow, Miriyala, Sumitra, St Clair, Daret, Craven, Rolf J
    Autophagy, 2013-09-04;9(10):1566-78.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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