Human Phospho-CEP250/CNAP1 (S2421) Antibody

Catalog #: MAB11339 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB11339-100
MAB11339-SP

Detection of Human CNAP1/CEP250 by Western Blot.

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All CNAP1/CEP250 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Phospho-CEP250/CNAP1 (S2421) Antibody Summary

Species Reactivity

Human

Specificity

Detects Human Phospho-CEP250/CNAP1 in direct ELISA

Source

Recombinant Monoclonal Rabbit IgG Clone # 2739C

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Phosphopeptide containing the human CEP250/CNAP1 S2421 site
Accession # Q9BV73

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

MDA‑MB‑231 human breast cancer cells and MDA-MB-231 + Calyculin A

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human CNAP1/CEP250 by Western Blot. Western blot shows lysates of MDA‑MB‑231 human breast cancer cells and MDA-MB-231 + Calyculin A. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human CNAP1/CEP250 Monoclonal Antibody (Catalog # MAB11339) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for CNAP1/CEP250 at approximately ~250 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CNAP1/CEP250

CEP250 (also known as CNAP1) is a large centrosome-associated protein that plays a crucial role in centrosome duplication and organization during cell division. Centrosomes are organelles play an essential role in cell division, cell polarity, and intracellular transport. In most animal cells, centrosomes consist of a pair of centrioles surrounded by pericentriolar material (PCM).CEP250/CNAP1 is a critical component of the PCM and is required for centrosome duplication and maturation. It forms a bridge between the centrioles and PCM and recruits other PCM components to the centrosome. Phosphorylation of CEP250/CNAP1 may regulate association/dissociation from centrosome. During M phase of mitosis, it is phosphorylated by NEK2, which may trigger the dissociation from the mitotic centrosome. This antibody detects CEP250/CNAP1 phosphorylated at Ser2421.

Long Name

Centrosomal Nek2-Associated Protein 1

Entrez Gene IDs

11190 (Human)

Alternate Names

250-KD; centrosomal Nek2-associated protein 1,250 kDa centrosomal protein; centrosomal protein 250kDa; centrosome associated protein; centrosome-associated protein CEP250; CEP2; CEP250; CNAP1; C-NAP1; MGC88542