Human Phospho-Chk2 (T68) Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Phospho-Chk2 (T68) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 1 hour. PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human Phospho-Chk2 (T68) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1626) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-Chk2 (T68) was detected at approximately 64 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda-phosphatase (lambda-PPase) for 60 minutes. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Phospho-Chk2 (T68) in HepG2 Human Cell Line. Phopsho-Chk2 (T68) was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line treated with 1 µM camptothecin using Rabbit Anti-Human Phospho-Chk2 (T68) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1626) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei in treated cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Chk2
ATM phosphorylates the Checkpoint kinase on T68 in response to genotoxic insults. Phosphorylation of T68 promotes the formation of Chk2 oligomers. Oligomerization is a critical event in the activation of the Chk2 kinase as it is required for phosphorylation of additional Chk2 residues that lead to full activation and signaling to downstream effectors.
Product Datasheets
Citations for Human Phospho-Chk2 (T68) Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
9
Citations: Showing 1 - 9
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Establishment and Characterization of Multi-Drug Resistant p53-Negative Osteosarcoma SaOS-2 Subline
Authors: Boichuk S, Bikinieva F, Valeeva E et al.
Diagnostics (Basel)
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Oocytes mount a noncanonical DNA damage response involving APC-Cdh1–mediated proteolysis
Authors: Goutham Narayanan Subramanian, Jessica Greaney, Zhe Wei, Olivier Becherel, Martin Lavin, Hayden Anthony Homer
Journal of Cell Biology
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WRN helicase is a synthetic lethal target in microsatellite unstable cancers
Authors: EM Chan, T Shibue, JM McFarland, B Gaeta, M Ghandi, N Dumont, A Gonzalez, JS McPartlan, T Li, Y Zhang, J Bin Liu, JB Lazaro, P Gu, CG Piett, A Apffel, SO Ali, R Deasy, P Keskula, RWS Ng, EA Roberts, E Reznichenk, L Leung, M Alimova, M Schenone, M Islam, YE Maruvka, Y Liu, J Roper, S Raghavan, M Giannakis, YY Tseng, ZD Nagel, A D'Andrea, DE Root, JS Boehm, G Getz, S Chang, TR Golub, A Tsherniak, F Vazquez, AJ Bass
Nature, 2019-04-10;568(7753):551-556.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Starvation-induced activation of ATM/Chk2/p53 signaling sensitizes cancer cells to cisplatin.
Authors: Shi Y, Felley-Bosco E, Marti T, Orlowski K, Pruschy M, Stahel R
BMC Cancer, 2012-12-04;12(0):571.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Multiple DNA damage signaling and repair pathways deregulated by simian virus 40 large T antigen.
Authors: Boichuk S, Hu L, Hein J
J. Virol., 2010-06-02;84(16):8007-20.
Species: Human
Sample Types: Whole Cells
Applications: Western Blot -
RAD51C facilitates checkpoint signaling by promoting CHK2 phosphorylation.
Authors: Badie S, Liao C, Thanasoula M, Barber P, Hill MA, Tarsounas M
J. Cell Biol., 2009-05-18;185(4):587-600.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Sonic Hedgehog signaling impairs ionizing radiation-induced checkpoint activation and induces genomic instability.
Authors: Leonard JM, Ye H, Wetmore C, Karnitz LM
J. Cell Biol., 2008-10-27;183(3):385-91.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Simian virus 40 large T antigen disrupts genome integrity and activates a DNA damage response via Bub1 binding.
Authors: Hein J, Boichuk S, Wu J, Cheng Y, Freire R, Jat PS, Roberts TM, Gjoerup OV
J. Virol., 2008-10-15;83(1):117-27.
Species: Human
Sample Types: Whole Cells
Applications: Western Blot -
RNF8-dependent and RNF8-independent regulation of 53BP1 in response to DNA damage.
Authors: Sakasai R, Tibbetts R
J. Biol. Chem., 2008-03-12;283(20):13549-55.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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Average Rating: 4.5 (Based on 2 Reviews)
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Excellent antibody, it worked extremely well for western blotting.
