Home / EphA2 / Human Phospho-EphA2 DuoSet IC ELISA

Human Phospho-EphA2 DuoSet IC ELISA

Catalog #: DYC4056-2
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DYC4056E
DYC4056-5
DYC4056-2
Ancillary Products Available
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
R&D Systems DuoSet ELISAs
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Citations (4)
FAQs
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Summary Product Features Kit Content Other Reagents Required Product Datasheets Preparation and Storage Background

Human Phospho-EphA2 DuoSet IC ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
Cell lysates (100 µL)
Sufficient Materials
Kits available for two, five, or fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure tyrosine-phosphorylated human EphA2 in cell lysates. An immobilized capture antibody specific for EphA2 binds both phosphorylated and unphosphorylated EphA2. After washing away unbound material, a biotinylated detection antibody is used to detect only phosphorylated receptor, utilizing a standard HRP format.

Product Features

  • Optimized capture and detection antibody pairings and recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Available in 2, 5, and 15-(96-well) plate pack sizes
  • Economical alternative to Western blot

Kit Content

  • Capture Antibody
  • Conjugated Detection Antibody
  • Calibrated Immunoassay Standard or Control

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Product Datasheets

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Product Datasheet
COA
SDS

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: EphA2

Cell migration is influenced by chemodirectants including a large family of receptor tyrosine kinases collectively referred to as Ephs. Ephs are divided into two classes based on their extracellular structure and ligand specificity. Eph receptors that bind preferentially to ephrin-A ligands are referred to as EphA. All Eph receptors contain an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail with one conserved tyrosine residue.

Entrez Gene IDs:
1969 (Human); 13836 (Mouse)
Alternate Names:
ARCC2; EC 2.7.10; EC 2.7.10.1; Eck; ECKepithelial cell receptor protein tyrosine kinase; EPH receptor A2; EphA2; ephrin type-A receptor 2; Epithelial cell kinase; Myk2; Sek2; soluble EPHA2 variant 1; Tyrosine-protein kinase receptor ECK

Citations for Human Phospho-EphA2 DuoSet IC ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Helicobacter Pylori Targets the EPHA2 Receptor Tyrosine Kinase in Gastric Cells Modulating Key Cellular Functions
    Authors: M Leite, MS Marques, J Melo, MT Pinto, B Cavadas, M Aroso, M Gomez-Laza, R Seruca, C Figueiredo
    Cells, 2020-02-24;9(2):.
    Species: Human
    Sample Types: Cell Culture Lysates
  2. Pinpointed Stimulation of EphA2 Receptors via DNA-Templated Oligovalence
    Authors: C Möser, JS Lorenz, M Sajfutdino, DM Smith
    Int J Mol Sci, 2018-11-06;19(11):.
    Species: Human
    Sample Types: Cell Lysates
  3. The ephrin receptor tyrosine kinase A2 is a cellular receptor for Kaposi's sarcoma-associated herpesvirus.
    Authors: Hahn AS, Kaufmann JK, Wies E, Naschberger E, Panteleev-Ivlev J, Schmidt K, Holzer A, Schmidt M, Chen J, Konig S, Ensser A, Myoung J, Brockmeyer NH, Sturzl M, Fleckenstein B, Neipel F
    Nat. Med., 2012-06-01;18(6):961-6.
    Species: Human
    Sample Types: Cell Lysates
  4. Lithocholic acid is an Eph-ephrin ligand interfering with Eph-kinase activation.
    Authors: Giorgio C, Hassan Mohamed I, Flammini L, Barocelli E, Incerti M, Lodola A, Tognolini M
    PLoS ONE, 2011-03-30;6(3):e18128.
    Species: Human
    Sample Types: Cell Lysates

FAQs

  1. Which phosphorylated sites are recognized by this assay?

    • This assay utilizes an anti-phosphorylated tyrosine monoclonal detection antibody, and it recognizes all phosphorylated tyrosine residues.

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