Home / EphB4 / Human Phospho-EphB4 DuoSet IC ELISA

Human Phospho-EphB4 DuoSet IC ELISA

Catalog #: DYC4057-2
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DYC4057-5
DYC4057-2
Ancillary Products Available
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
R&D Systems DuoSet ELISAs
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Citations (5)
FAQs
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Summary Product Features Kit Content Other Reagents Required Product Datasheets Preparation and Storage Background

Human Phospho-EphB4 DuoSet IC ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
Cell lysates (100 µL)
Sufficient Materials
Kits available for two, five, or fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure tyrosine-phosphorylated human EphB4 in cell lysates. An immobilized capture antibody specific for EphB4 binds both phosphorylated and unphosphorylated EphB4. After washing away unbound material, a biotinylated detection antibody is used to detect only phosphorylated receptor, utilizing a standard HRP format.

Product Features

  • Optimized capture and detection antibody pairings and recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Available in 2, 5, and 15-(96-well) plate pack sizes
  • Economical alternative to Western blot

Kit Content

  • Capture Antibody
  • Conjugated Detection Antibody
  • Calibrated Immunoassay Standard or Control

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Product Datasheets

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Product Datasheet
COA
SDS

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: EphB4

Cell migration is influenced by chemodirectants including a large family of receptor tyrosine kinases collectively referred to as Ephs. Ephs are divided into two classes based on their extracellular structure and ligand specificity. Eph receptors that bind preferentially to ephrin-B ligands are referred to as EphB. All Eph receptors contain an N-terminal Ig-like domain, a cysteine-rich region with 19 conserved cysteines and two fibronectin type III domains. The cytoplasmic region contains a typical tyrosine kinase organization.

Long Name:
Eph Receptor B4
Entrez Gene IDs:
2050 (Human); 13846 (Mouse)
Alternate Names:
EC 2.7.10; EC 2.7.10.1; EPH receptor B4; EphB4; ephrin type-B receptor 4; hepatoma transmembrane kinase; Htk; HTKephrin receptor EphB4; Mdk2; Myk1; soluble EPHB4 variant 1; soluble EPHB4 variant 2; soluble EPHB4 variant 3; Tyro11; Tyrosine-protein kinase receptor HTK; Tyrosine-protein kinase TYRO11

Citations for Human Phospho-EphB4 DuoSet IC ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Manipulating the EphB4-ephrinB2 axis to reduce metastasis in HNSCC
    Authors: Abdelazeem, KNM;Nguyen, D;Corbo, S;Darragh, LB;Matsumoto, MW;Van Court, B;Neupert, B;Yu, J;Olimpo, NA;Osborne, DG;Gadwa, J;Ross, RB;Nguyen, A;Bhatia, S;Kapoor, M;Friedman, RS;Jacobelli, J;Saviola, AJ;Knitz, MW;Pasquale, EB;Karam, SD;
    Oncogene
    Species: Human
    Sample Types: Cell Lysates
  2. EphB4 forward signalling mediates angiogenesis caused by CCM3/PDCD10-ablation
    Authors: C You, K Zhao, P Dammann, K Keyvani, I Kreitschma, U Sure, Y Zhu
    J. Cell. Mol. Med., 2017-04-01;0(0):.
    Species: Human
    Sample Types:
  3. Enhancing radiosensitization in EphB4 receptor-expressing Head and Neck Squamous Cell Carcinomas
    Sci Rep, 2016-12-12;6(0):38792.
    Species: Human
    Sample Types: Tissue Homogenates
  4. Lithocholic acid is an Eph-ephrin ligand interfering with Eph-kinase activation.
    Authors: Giorgio C, Hassan Mohamed I, Flammini L, Barocelli E, Incerti M, Lodola A, Tognolini M
    PLoS ONE, 2011-03-30;6(3):e18128.
    Species: Human
    Sample Types: Cell Lysates
  5. The ephrinB2/EphB4 axis is dysregulated in osteoprogenitors from myeloma patients and its activation affects myeloma bone disease and tumor growth.
    Authors: Pennisi A, Ling W, Li X, Khan S, Shaughnessy JD, Barlogie B, Yaccoby S
    Blood, 2009-07-13;114(9):1803-12.
    Species: Human
    Sample Types: Cell Lysates

FAQs

  1. Which phosphorylated sites are recognized by this assay?

    • This assay utilizes an anti-phosphorylated tyrosine monoclonal detection antibody, and it recognizes all phosphorylated tyrosine residues.

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