Human Phospho-FAK (Y397) Antibody Summary
Accession # Q05397
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Phospho-FAK (Y397) by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 0.1 mM Pervanadate (PV) for 10 minutes. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human Phospho-FAK (Y397) Monoclonal Antibody (Catalog # MAB4528) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-FAK (Y397) at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FAK
Focal adhesion kinase 1 (FAK) is a ubiquitously expressed non-receptor protein tyrosine kinase that is concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. This cellular localization is directed by a "Focal Adhesion Targeting" (FAT) sequence, a 125 amino acid sequence at the C-terminus. FAK plays an important role in migration, cell spreading, differentiation, cytoskeleton protein phosphorylation, apoptosis and acceleration of the G1 to S phase transition of the cell cycle. It associates with several different signaling proteins such as Src-family PTKs, p130Cas, Shc, Grb2, PI 3-kinase, and paxillin. This enables FAK to function within a network of integrin-stimulated signaling pathways leading to the activation of targets such as the ERK and JNK/mitogen-activated protein kinase pathways. FAK is also linked to oncogenes at biochemical and functional levels. Increased expression and/or activity of FAK in various tumors has been correlated with enhanced migration and invasiveness of human tumor cells in addition to promoting increased cell proliferation.
Product Datasheets
Citations for Human Phospho-FAK (Y397) Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Insight into Cisplatin-Resistance Signaling of W1 Ovarian Cancer Cells Emerges mTOR and HSP27 as Targets for Sensitization Strategies
Authors: K Wantoch vo, P König, S Henze, M Schlesinge, P Zawierucha, R Januchowsk, G Bendas
International Journal of Molecular Sciences, 2020-12-03;21(23):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
FAK inhibition reduces metastasis of alpha 4 integrin-expressing melanoma to lymph nodes by targeting lymphatic VCAM-1 expression
Authors: Kyuho Jeong, James M. Murphy, Yelitza A.R. Rodriguez, Jun-Sub Kim, Eun-Young Erin Ahn, Ssang-Taek Steve Lim
Biochemical and Biophysical Research Communications
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Clonorchis sinensis excretory-secretory products promote the migration and invasion of cholangiocarcinoma cells by activating the integrin ?4-FAK/Src signaling pathway
Authors: JH Pak, Q Bashir, IK Kim, SJ Hong, S Maeng, YY Bahk, TS Kim
Mol. Biochem. Parasitol, 2017-03-08;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Kaposi's sarcoma-associated herpesvirus induces rapid release of angiopoietin-2 from endothelial cells.
Authors: Ye F, Zhou F, Nithianantham S, Chandran B, Yu X, Weinberg A, Gao S
J Virol, 2013-03-27;87(11):6326-35.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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Mouse pancreatic cancer cells were implanted in C57 BL/6 syngeneic host for 1 month
Tumor tissue was fixed in 4% PFA in PBS overnight in cold
Then equilibriated in 30% sucrose in PBS in cold overnight
Embedded in OCT and sectioned at 10um
Stain with antibody for 2h after blocking with Fc recerptor
Counterstained with DAPI
