Human Phospho-GCN2 (T899) Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Phospho-GCN2 (T899) by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 20 mJ/cm2ultraviolet light (UV) with a 30 minute recovery. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-GCN2 (T899) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7605) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-GCN2 (T899) at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GCN2
GCN2 (General Control Non-derepressible 2-like protein; also eIF2a kinase 4) is a 190-200 kDa member of the GCN2 subfamily, Ser/Thr kinase family, protein kinase superfamily of proteins It is widely expressed, being found in skeletal muscle cells, fibroblasts, hepatocytes and neurons. GCN2 is a key regulator of metabolism under nutrient deprivation. In particular, when essential amino acids (aa) are limiting, tRNAs exist that are unbound to amino acids. These “uncharged” tRNAs will bind to GCN2, activating the molecule. Through GCN2-mediated phosphorylation of eIF2a, promotes GCN4 synthesis, with subsequent activation of amino acid synthesizing enzymes, and a downregulation of lipid storage. Human GCN2 is 1649 aa in length. It possesses one RWD domain (aa 25-137), two protein kinase domains (aa 296-539 and 590-1001) and an extended His-RS-like region (aa 1021-1492). There are at least three utilized Ser/Thr phosphorylation sites. Two isoform variants exist. One shows a deletion of aa 774-801, while another contains a nine aa substitution for aa 608-1649. Over aa 22-139, human GCN2 shares 91% aa sequence identity with mouse GCN2.
Product Datasheets
Citations for Human Phospho-GCN2 (T899) Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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The GCN2/eIF2 alpha K stress kinase regulates PP1 to ensure mitotic fidelity
Authors: Vilte Stonyte, Maria Mastrangelopoulou, Romy Timmer, Lilian Lindbergsengen, Marina Vietri, Coen Campsteijn et al.
EMBO reports
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Immunogenic cell death triggered by impaired deubiquitination in multiple myeloma relies on dysregulated type I interferon signaling
Authors: Zeinab Waad Sadiq, Annamaria Brioli, Ruba Al-Abdulla, Gonca Çetin, Jacqueline Schütt, Hugo Murua Escobar et al.
Frontiers in Immunology
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