Human Phospho-IFN-alpha / beta R1 (S535/S539) Antibody

Catalog #: PPS074 Datasheet / COA / SDS

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PPS074

Detection of Phospho-IFN-alpha / beta R1 (S535/S539) by Western Blot

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Human Phospho-IFN-alpha / beta R1 (S535/S539) Antibody Summary

Species Reactivity

Human

Specificity

Human IFN-alpha R1, Type I, Subunit I phosphorylated at S535/S539

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-S535/S539 of IFN-alpha R1 Subunit I

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1:1000 dilution HEK293 human embryonic kidney cell line transfected with IFN-alpha R1

Note: The molecular weight of the IFN-alpha R1 varies with cell line (different levels of glycosylation) in HEK293 and HeLa cells; the mature form is ~110 - 130 kDa.

Immunohistochemistry

1:1000 dilution

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Phospho-IFN-alpha / beta R1 (S535/S539) by Western Blot Western blot of immunoprecipitates from HEK293 cells transfected with 1) Mock, 2) IFN-alpha R1 wild type, and 3) IFN-alpha R1 S535A and S539A mutants showing specific immunolabeling of the ~110 kDa to ~130 kDa IFN-alpha R1 wild type. The immunolabeling is absent in the IFN-alpha R1 S535 and S539 mutants (Control). The immunolableing is blocked by the phosphopeptide (Phos) used as the antigen but not by the corresponding dephosphopeptide (Dephos).

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: IFN-alpha/beta R1

Interferons are widely used therapeutic agents because of their anti tumor and antiviral effects and because of their modulatory effects on the immune system (1,2). These cytokines produce their effects by binding to the Type I IFN Receptor. This receptor contains two subunits; IFNAR1/ alpha 1 and IFNAR2/ alpha 2 Down regulation of this receptor plays a key role in determining the magnitude and duration of cytokine signaling. This downregulation is thought to be influenced by phosphorylation of S535 and S539 in IFN-alpha R1 (3).

References

Biron, C.A. (2001) Immunity 14:661.
Kirkwood, J. (2002) Semin. Oncol. 29:18.
Kumar, K.G. et al. (2003) EMBO J. 22:5480.

Long Name

Interferon alpha/beta Receptor 1

Entrez Gene IDs

3454 (Human); 15975 (Mouse); 101926190 (Cynomolgus Monkey)

Alternate Names

alpha-type antiviral protein; AVP; beta-type antiviral protein; CRF2-1; Cytokine receptor class-II member 1; Cytokine receptor family 2 member 1; human interferon-alpha receptor (HuIFN-alpha-Rec)10IFRC; IFN-alpha/beta R1; IFN-alpha/beta receptor 1; IFN-alpha-REC; IFNAR; IFNAR1; IFN-aR1; IFNBR; IFNbR1; IFN-bR1; IFN-R-1; interferon (alpha, beta and omega) receptor 1; interferon alpha/beta receptor 1; interferon-alpha/beta receptor alpha chain; interferon-beta receptor 1; Type I interferon receptor 1