Human Phospho-MEK1 (T292) Antibody

Name: Human Phospho-MEK1 (T292) Antibody
Brand: R&D Systems
SKU: PPS075

Catalog #: PPS075 Datasheet / COA / SDS

Discontinued Product

PPS075 has been discontinued.

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Detection of Phospho-MEK1 (T292) by Western Blot

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Human Phospho-MEK1 (T292) Antibody Summary

Species Reactivity

Human

Specificity

Human ~45 kDa MEK1 phosphorylated at T292

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding human phospho-T292 of MEK1

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1:1000 dilution

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Phospho-MEK1 (T292) by Western Blot Western blot of recombinant Wild Type (WT) and mutant MEK1 with and without coexpression of MAP Kinase showing immunolabeling of the ~45 kDa MEK1 protein phosphorylated at T292.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: MEK1

Human MEK1 (MAPK/ERK kinase 1) is a 45 kDa member of the MAP kinase kinase subfamily of the Ser/Thr protein kinase family of enzymes. It is 393 amino acids (aa) in length and contains one ERK interaction (aa 1 - 32) and catalytic site (aa 68 - 361). There is one alternate splice form that shows a 26 aa deletion between aa 471 - 548. MEK1 is part of the MAP kinase pathway that is activated in response to growth factors and integrins. Two signal transduction pathways converge on MEK1. Mitogens activate Ras, which activates Raf, which activates MEK1. Integrins activate Rac, which activates PAK, which also activates MEK1. Raf activates MEK1 by phosphorylating S218 and S222 on MEK1. Alternatively, PAK indirectly activates MEK1 by phosphorylating MEK1 on S298, which promotes the Raf phosphorylation of S218 and S222. Once activated, MEK1 phosphorylates and activates ERK1/2. In a negative feedback loop, activated ERK can phosphorylate MEK1 on T292 and T386. This phosphorylation of T292 will interfere with PAK phosphorylation of MEK1 on S298. ERK-mediated phosphorylation of MEK1 at T386 may also interfere with PAK phosphorylation of MEK1.

References

Chong, H. et al. (2003) Cell. Signal. 15:463.
Eblen, S.T. et al. (2004) Mol. Cell. Biol. 24:2308.
Xu, B. et al. (1999) J. Biol. Chem. 274:34029.
Frost, J.A. et al. (1997) EMBO J. 16:6426.
Mansour, S.J. et al. (1994) J. Biochem. 116:304.
Zheng, C-F. and K-L. Guan (1994) EMBO J. 13:1123.

Long Name

Mitogen-activated Protein Kinase Kinase 1

Entrez Gene IDs

5604 (Human); 26395 (Mouse)

Alternate Names

dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAP kinase kinase 1; MAP2K1; MAPK/ERK kinase 1; MAPKK1; MEK1; MEK1MAPKK 1; mitogen-activated protein kinase kinase 1; MKK1; MKK1MEK 1; PRKMK1EC 2.7.12.2; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)