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Human Phospho-Nbs1 (S343) Antibody

Catalog #: AF4944 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4944
AF4944-SP
Detection of Human Phospho-Nbs1 (S343) by Western Blot.
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Human Phospho-Nbs1 (S343) Antibody Summary

Species Reactivity
Human
Specificity
Detects human Nbs1 when phosphorylated at S343. Does not recognize Nbs1 when unphosphorylated at S343.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Phosphopeptide containing human Nbs1 S343 site
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS and Sodium Azide with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Phospho-Nbs1 (S343) antibody by Western Blot. View Larger

Detection of Human Phospho-Nbs1 (S343) by Western Blot. Western blot shows Nbs1 immunoprecipitated from lysates of HeLa human cervical epithelial carcinoma cell line using Human Nbs1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1573). HeLa cell line was mock treated or exposed to 100 J/m2UV-C for 3 hours. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human Phospho-Nbs1 (S343) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4944) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-Nbs1 (S343) was detected at approximately 95 kDa (as indicated). For additional reference the membrane was stripped and reprobed with 1 µg/mL Human/Mouse/Rat Nbs1 Monoclonal Antibody (lower panel, Catalog # MAB1573) This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nbs1

The Nijmegen Breakage Syndrome 1 (Nbs1) protein is a member of the Mre11/Rad50/Nbs1 (MRN) protein complex that binds to DNA double-strand breaks in cells exposed to DNA damaging agents. In addition, the MRN complex colocalizes with replication forks during DNA replication. The MRN complex plays an important role in routine cell cycle progression and genotoxic stress responses by facilitating DNA repair. Nbs1 is phosphorylated at S343 by ATM in response to double-strand breaks and by ATR under replication stress. This phosphorylation triggers the inactivation of late origin firing, which is essential for mediating the intra-S-phase checkpoint. Mutation of the nbs1 gene and resultant loss of Nbs1 protein expression in humans results in the chromosomal instability disease, Nijmegen Breakage Syndrome.

Long Name
Nijmegen Breakage Syndrome 1
Entrez Gene IDs
4683 (Human); 27354 (Mouse); 85482 (Rat)
Alternate Names
ATV; AT-V1; AT-V2; Cell cycle regulatory protein p95; FLJ10155; MGC87362; NBN; NBS; Nbs1; NBS1P95; Nibrin; Nijmegen breakage syndrome 1 (nibrin); Nijmegen breakage syndrome protein 1; p95 protein of the MRE11/RAD50 complex; p95

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