Home / p70 S6 Kinase / Human Phospho-p70 S6 Kinase (T229) Antibody

Human Phospho-p70 S6 Kinase (T229) Antibody

Catalog #: MAB8964
2 Citations Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB8964
MAB8964-SP
Detection of Human Phospho-p70 S6 Kinase (T229) by Western Blot.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Phospho-p70 S6 Kinase (T229) Antibody Summary

Species Reactivity
Human
Specificity
Detects human p70 S6 Kinase when phosphorylated at T229.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1015B
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Phosphopeptide containing human p70S6K T229
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Immunocytochemistry
2.5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Phospho-p70 S6 Kinase (T229) antibody by Western Blot. View Larger

Detection of Human Phospho-p70 S6 Kinase (T229) by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF-I (Catalog # 291-G1) for 1 hour. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-p70 S6 Kinase (T229) Monoclonal Antibody (Catalog # MAB8964) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-p70 S6 Kinase (T229) at approximately 70 and 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p70 S6 Kinase

p70 S6 Kinase (p70S6K) is responsible for the phosphorylation of 40S ribosomal protein S6 and is ubiquitously expressed in human adult tissues (1). p70S6K is activated by serum stimulation and this activation is inhibited by wortmannin and rapamycin. p70S6K activity undergoes changes in the cell cycle and increases 20-fold in G1 cells released from G0 (2). p70S6K activation requires sequential phosphorylations at proline-directed residues in the putative autoinhibitory pseudosubstrate domain, as well as T389, a site phosphorylated by Phosphoinositide-Dependent Kinase 1 (PDK1).

References
  1. Ferrari, S. et al. (1994) Crit. Rev. Biochem. Mol. Biol. 29:385.
  2. Edelmann, H.M. et al. (1996) J. Biol. Chem. 271:963.
Entrez Gene IDs
6198 (Human); 72508 (Mouse); 83840 (Rat)
Alternate Names
EC 2.7.11; EC 2.7.11.1; p70 ribosomal S6 kinase alpha; p70 S6 kinase alpha; p70 S6 Kinase; p70 S6 kinase, alpha 1,70 kDa ribosomal protein S6 kinase 1; p70 S6 kinase, alpha 2; p70 S6KA; p70 S6K-alpha; p70(S6K)-alpha; p70-alpha; p70-S6K; P70S6K1; PS6K; ribosomal protein S6 kinase beta-1; Ribosomal protein S6 kinase I; ribosomal protein S6 kinase, 70kD, polypeptide 1; ribosomal protein S6 kinase, 70kDa, polypeptide 1; RPS6KB1; S6K; S6K1; S6K1p70-S6K 1; S6K-beta-1; serine/threonine kinase 14 alpha; Serine/threonine-protein kinase 14A; STK14A

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Citations for Human Phospho-p70 S6 Kinase (T229) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Role of PDK1 in skeletal muscle hypertrophy induced by mechanical load
    Authors: N Kuramoto, K Nomura, D Kohno, T Kitamura, G Karsenty, T Hosooka, W Ogawa
    Scientific Reports, 2021-02-10;11(1):3447.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Serine 389 phosphorylation of 3-phosphoinositide-dependent kinase 1 by UNC-51-like kinase 1 affects its ability to regulate Akt and p70 S6kinase
    Authors: Kidae Kim, Sung Goo Park, Byoung Chul Park, Jeong-Hoon Kim, Sunhong Kim
    BMB Reports

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