Human Phospho-p70 S6 Kinase (T229) Antibody
Human Phospho-p70 S6 Kinase (T229) Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Phospho-p70 S6 Kinase (T229) and p85 S6 Kinase (T252) by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human EGF (Catalog # 236-EG) for 15 minutes or 100 ng/mL Recombinant Human IGF-1 (Catalog # 291-G1) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Human Phospho-p70 S6 Kinase (T229) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF896), followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for Phospho-p70 S6 Kinase (T229) and p85 S6 Kinase (T252) at approximately 70 and 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Phospho-p70 S6 Kinase (T229) in MCF‑7 Human Cell Line. p70 S6 Kinase phosphorylated at T229 was detected in immersion fixed MCF-7 human breast cancer cell line treated with Recombinant Human IGF-I (Catalog # 291-G1) using Human Phospho-p70 S6 Kinase (T229) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF896) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: p70 S6 Kinase
p70 S6 kinase is a Ser/Thr kinase activated by such mitogens as EGF, IGF-I, and insulin. The S6 protein of the 40S ribosomal subunit is a major substrate of p70 S6 kinase, and its phosphorylation upregulates the translation of mRNAs containing an oligopyrimidine tract at their transcriptional start site. The activity of p70 S6 kinase is controlled by multiple phosphorylation events, with phosphorylation at T229 by PDK1 and T389 by mTOR most critical for kinase function.
Product Datasheets
Citation for Human Phospho-p70 S6 Kinase (T229) Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Diacylglycerol kinase delta modulates Akt phosphorylation through pleckstrin homology domain leucine-rich repeat protein phosphatase 2 (PHLPP2).
Authors: Crotty T, Nakano T, Stafforini D, Topham M
J Biol Chem, 2012-11-26;288(3):1439-47.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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