Human Phospho-Smad2/3 (S465/S467) Antibody

Name: Human Phospho-Smad2/3 (S465/S467) Antibody
Brand: R&D Systems
SKU: MAB8935
Rating: 5 (1 reviews)

Catalog #: MAB8935
11 Citations 1 Review Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB8935
MAB8935-SP

Detection of Human Phospho-Smad2/3 (S465/S467) by Western Blot.

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Product Details

Citations (11)

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Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Phospho-Smad2/3 (S465/S467) Antibody Summary

Species Reactivity

Human

Specificity

Detects human Smad2 and Smad3 when dually phosphorylated at S465 and S467 in Western blots.

Source

Recombinant Monoclonal Rabbit IgG Clone # 1074A

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Phosphopeptide containing the human Smad2 S465/S467 site
Accession # NP_005892

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Phospho-Smad2/3 (S465/S467) by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 10 ng/mL Recombinant Human TGF-beta 1 (Catalog # 240-B) for 30 minutes. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human Phospho-Smad2/3 (S465/S467) Monoclonal Antibody (Catalog # MAB8935) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Smad2/3 (S465/S467) at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Smad2/3

Smads are a family of intracellular proteins that transmit transforming growth factor beta (TGF-beta ) superfamily signals from the cell surface to the nucleus. The Smad family is divided into three subclasses: receptor regulated Smads, (Smads 1, 2, 3, 5 and 8); the common partner, (Smad4); and the inhibitory Smads, (Smads 6 and 7). The binding of TGF-beta or activin to their cognate receptor induces phosphorylation of Smads 2 and 3. The activated Smads associate with the common-mediator subunit, Smad4, and the heteromeric complex translocates into the nucleus to initiate transcription. Smad3, also known as Mothers Against Decapentaplegic homolog 3 (MADH3), shares 83% amino acid identity with Smad2, also known as Mothers Against Decapentaplegic homolog 2 (MADH2). Human Smad2 has 99% identity to mouse and rat Smad2. Human Smad3 has 99% identity to mouse and rat Smad3.

Long Name

Mothers Against DPP Homolog 2/3

Alternate Names

hMAD-2; hSMAD2; JV18; JV18-1SMAD, mothers against DPP homolog 2 (Drosophila); MAD homolog 2; Mad protein homolog; Mad, mothers against decapentaplegic homolog 2; MADH2MGC34440; MADR2MGC22139; Mad-related protein 2; mother against DPP homolog 2; mothers against decapentaplegic homolog 2; Mothers against DPP homolog 2; Sma- and Mad-related protein 2; SMAD 2; SMAD family member 2MAD, mothers against decapentaplegic homolog 2 (Drosophila); SMAD, mothers against DPP homolog 2; Smad2; Smad2/3

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Phospho-Smad2/3 (S465/S467) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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DPP-4 Inhibitors Attenuate Fibrosis After Glaucoma Filtering Surgery by Suppressing the TGF-?/Smad Signaling Pathway
Authors: Yoshida M, Kokubun T, Sato K et al.
Investigative ophthalmology & visual science
Cadherin-11 Influences Differentiation in Human Mesenchymal Stem Cells by Regulating the Extracellular Matrix Via the TGF?1 Pathway
Authors: Passanha FR, Geuens T, LaPointe VLS
Stem cells (Dayton, Ohio)
Soluble Fraction from Lysate of a High Concentration Multi-Strain Probiotic Formulation Inhibits TGF-&beta1-Induced Intestinal Fibrosis on CCD-18Co Cells
Authors: F Lombardi, FR Augello, P Palumbo, E Mollsi, M Giuliani, AM Cimini, MG Cifone, B Cinque
Nutrients, 2021-03-09;13(3):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Immune modulation by complement receptor 3-dependent human monocyte TGF-beta1-transporting vesicles
Authors: LD Halder, EAH Jo, MZ Hasan, M Ferreira-G, T Krüger, M Westermann, DI Palme, G Rambach, N Beyersdorf, C Speth, ID Jacobsen, O Kniemeyer, B Jungnickel, PF Zipfel, C Skerka
Nat Commun, 2020-05-11;11(1):2331.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Modeling Progressive Fibrosis with Pluripotent Stem Cells Identifies an Anti-fibrotic Small Molecule
Authors: P Vijayaraj, A Minasyan, A Durra, S Karumbayar, M Mehrabi, CJ Aros, SD Ahadome, DW Shia, K Chung, JM Sandlin, KF Darmawan, KV Bhatt, CC Manze, MK Paul, DC Wilkinson, W Yan, AT Clark, TM Rickabaugh, WD Wallace, TG Graeber, R Damoiseaux, BN Gomperts
Cell Rep, 2019-12-10;29(11):3488-3505.e9.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot
CD109 acts as a gatekeeper of the epithelial trait by suppressing epithelial to mesenchymal transition in squamous cell carcinoma cells in vitro
Authors: S Zhou, SD da Silva, PM Siegel, A Philip
Sci Rep, 2019-11-06;9(1):16317.
Species: Human
Sample Types: Protein
Applications: Western Blot
Label-free quantitative proteomics identifies transforming growth factorÎ²1 (TGF-?1) as an inhibitor of adipogenic transformation in OP9-DL1 cells and primary thymic stromal cells
Authors: J Tan, Y Wang, S Wang, S Wu, Z Yuan, X Zhu
Cell Biosci, 2019-06-14;9(0):48.
Species: Mouse
Sample Types: Cell Lysates
Applications: ChIP
COA-Cl prevented TGF-?1-induced CTGF expression by Akt dephosphorylation in normal human dermal fibroblasts, and it attenuated skin fibrosis in mice models of systemic sclerosis
Authors: K Nakai, S Karita, J Igarashi, I Tsukamoto, K Hirano, Y Kubota
J. Dermatol. Sci., 2019-03-12;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Neuronal Protein 3.1 Deficiency Leads to Reduced Cutaneous Scar Collagen Deposition and Tensile Strength due to Impaired Transforming Growth Factor-?1 to -?3 Translation
Authors: Tao Cheng
Am. J. Pathol, 2016-12-08;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Suppressive effect mediated by human adipose-derived stem cells on T cells involves the activation of JNK
Authors: Yinmin Wang, Xiuxia Wang, Xianyu Zhou, Zhu Zhu, Jun Yang, Fei Liu
International Journal of Molecular Medicine

Hypoxia-induced TGF-beta –RBFOX2–ESRP1 axis regulates human MENA alternative splicing and promotes EMT in breast cancer
Authors: Neha Ahuja, Cheemala Ashok, Subhashis Natua, Deepak Pant, Anna Cherian, Madhura R Pandkar et al.
NAR Cancer