Human Phospho-STAT3 (Y705) Antibody

Catalog #: MAB4607
5 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4607
MAB4607-SP

Detection of Human Phospho‑STAT3 (Y705) by Western Blot.

1 Image

All STAT3 Antibodies

Product Details

Citations (5)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Phospho-STAT3 (Y705) Antibody Summary

Species Reactivity

Human

Specificity

Detects human STAT3 when phosphorylated at Y705.

Source

Monoclonal Rat IgG_2B Clone # 805435

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Phosphopeptide containing the human STAT3 Y705 site

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human Phospho‑STAT3 (Y705) by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 500 U/mL Recombinant Human IFN-aA (Catalog # 11100-1) for 20 minutes or 50 µg/mL Recombinant Human IL-6 (Catalog # 206-IL) for 15 minutes. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human Phospho-STAT3 (Y705) Monoclonal Antibody (Catalog # MAB4607) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-STAT3 (Y705) at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: STAT3

Human STAT-3/STAT3a (signal transducer and activator of transcription 3; also called APRF) is a 90-95 kDa member of the STAT family of transcription factors. It is 770 amino acids (aa) in length, contains one SH2 domain (aa 580-670), and is found in almost all cell types. STAT3 mediates gp130, LIF-R, OB-R, IL10-R and EGFR signaling. Upon activation, receptors such as gp130 phosphorylate STAT3 at Ser727, which results in transcriptional activation. Phosphorylation at Tyr705 mediates homo- and hetero-dimerization with STAT1 and nuclear translocation, and is an important event in oncogenic transformation. The STAT3b isoform shows a six aa substitution for aa 716-770, thus eliminating the Ser727 site. Human and mouse STAT3a share 99% aa sequence identity.

Long Name

Signal Transducer and Activator of Transcription 3

Entrez Gene IDs

6774 (Human); 20848 (Mouse); 25125 (Rat)

Alternate Names

Acute-phase response factor; APRFMGC16063; DNA-binding protein APRF; FLJ20882; HIES; signal transducer and activator of transcription 3 (acute-phase responsefactor); signal transducer and activator of transcription 3; STAT3

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Phospho-STAT3 (Y705) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
Filter your results:

Filter by:

Effects of Tofacitinib on Muscle Remodeling in Experimental Rheumatoid Sarcopenia
Authors: Bermejo-Álvarez, I;Pérez-Baos, S;Gratal, P;Medina, JP;Largo, R;Herrero-Beaumont, G;Mediero, A;
International journal of molecular sciences
Species: Rabbit
Sample Types: Tissue Homogenates
Applications: Western Blot
Thymosin ?4 Alleviates Autoimmune Dacryoadenitis via Suppressing Th17 Cell Response
Authors: Zhao, X;Li, N;Yang, N;Mi, B;Dang, W;Sun, D;Ma, S;Nian, H;Wei, R;
Investigative ophthalmology & visual science
Species: Rabbit
Sample Types: Cell Lysates
Applications: Western Blot
Tumor-derived mesenchymal-stem-cell-secreted IL-6 enhances resistance to cisplatin via the STAT3 pathway in breast cancer
Authors: H Xu, Y Zhou, W Li, B Zhang, H Zhang, S Zhao, P Zheng, H Wu, J Yang
Oncol Lett, 2018-04-11;15(6):9142-9150.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Compensatory anabolic signaling in the sarcopenia of experimental chronic arthritis
Authors: RD Little, I Prieto-Pot, S Pérez-Baos, A Villalvill, P Gratal, F Cicuttini, R Largo, G Herrero-Be
Sci Rep, 2017-07-24;7(1):6311.
Species: Rabbit
Sample Types: Tissue Homogenates
Applications: Western Blot
Inhibition of pSTAT1 by tofacitinib accounts for the early improvement of experimental chronic synovitis
Authors: Sandra Pérez-Baos, Paula Gratal, Juan I. Barrasa, Ana Lamuedra, Olga Sánchez-Pernaute, Gabriel Herrero-Beaumont et al.
Journal of Inflammation