Home / STAT4 / Human Phospho-STAT4 (Y693) Antibody

Human Phospho-STAT4 (Y693) Antibody

Catalog #: AF4319
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4319
AF4319-SP
Detection of Human Phospho-STAT4 (Y693) by Western Blot.
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Citations (4)
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Human Phospho-STAT4 (Y693) Antibody Summary

Species Reactivity
Human
Specificity
Detects human STAT4 when phosphorylated at Y693.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Phosphopeptide containing human Phospho-STAT4 Y693 site
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Phospho-STAT4 (Y693) antibody by Western Blot. View Larger

Detection of Human Phospho-STAT4 (Y693) by Western Blot. Western blot shows lysates of NC-37 human Burkitt's lymphoma B lymphoblast cell line untreated (-) or treated (+) with pervanadate for 5 minutes. PVDF membrane was probed with 1 µg/mL Human Phospho-STAT4 (Y693) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4319) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-STAT4 (Y693) was detected at approximately 95 kDa (as indicated). For additional reference, duplicate lysates were probed with 1.0 µg/mL Human/Mouse STAT4 Polyclonal Antibody (lower panel, Catalog # PA-ST4). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: STAT4

Signal transduction and activator of transcription 4 (STAT4) is a member of the Jak/STAT signal transduction pathway. STAT4 mediates cytokine signaling by acting as a signal transducer in the cytoplasm and, upon phosphorylation, homo or heterodimerizes with other STAT proteins, which then translocate to the nucleus and activate transcription of specific genes. STAT4 is expressed in subset of tissues including spleen, heart, brain, peripheral blood cells and testis. STAT4 is involved in mediating IL-12 response in lymphocytes and regulating T helper cell differentiation.

Long Name
Signal Transducer and Activator of Transcription 4
Entrez Gene IDs
6775 (Human); 20849 (Mouse)
Alternate Names
signal transducer and activator of transcription 4; SLEB11; STAT4

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Citations for Human Phospho-STAT4 (Y693) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Human memory, but not naive, CD4+ T cells expressing transcription factor T-bet might drive rapid cytokine production.
    Authors: Yu S, Zhang Y, Yang B, Wu C
    J Biol Chem, 2014-11-05;289(51):35561-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Human invariant natural killer T cells acquire transient innate responsiveness via histone H4 acetylation induced by weak TCR stimulation.
    Authors: Wang X, Bishop KA, Hegde S, Rodenkirch LA, Pike JW, Gumperz JE
    J. Exp. Med., 2012-04-16;209(5):987-1000.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Novel immunomodulatory properties of berbamine through selective down-regulation of STAT4 and action of IFN-gamma in experimental autoimmune encephalomyelitis.
    Authors: Ren Y, Lu L, Guo TB, Qiu J, Yang Y, Liu A, Zhang JZ
    J. Immunol., 2008-07-15;181(2):1491-8.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Native CGRP Neuropeptide and Its Stable Analogue SAX, But Not CGRP Peptide Fragments, Inhibit Mucosal HIV-1 Transmission
    Authors: Jammy Mariotton, Anette Sams, Emmanuel Cohen, Alexis Sennepin, Gabriel Siracusano, Francesca Sanvito et al.
    Frontiers in Immunology

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