Human PIEZO1 Antibody

Catalog #: MAB11557 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11557-100
MAB11557-SP

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Human PIEZO1 Antibody Summary

Species Reactivity

Human

Specificity

Detects a synthetic peptide around aa 150 in Direct ELISA

Source

Monoclonal Mouse IgG_2B Clone # 1081418

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

PIEZO1 containing synthetic peptide
Accession # Q92508

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

3-25 µg/mL

Immersion fixed paraffin-embedded sections of human kidney

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Detection of PIEZO1 in Human Kidney. PIEZO1 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human PIEZO1 Monoclonal Antibody (Catalog # MAB11557) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute lyophilized material at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PIEZO1

Piezo1 is an 287kDa mechanosensitive nonselective cation channel, extensively distributed in multiple tissues and cell types. Piezo1 plays an important role in numerus physiological processes, such as determining vascular structure, urine osmoregulation, and blood pressure homeostasis. In the kidney, Piezo1 expression is found in the renal corpuscle, convoluted tubules, and collecting ducts.

References

Zhao X, Kong Y, Liang B, Xu J, Lin Y, Zhou N, Li J, Jiang B, Cheng J, Li C, Wang W. Mechanosensitive Piezo1 channels mediate renal fibrosis. JCI Insight. 2022 Apr 8;7(7):e152330. doi: 10.1172/jci.insight.152330. PMID: 35230979; PMCID: PMC9057604.
Carrisoza-Gaytan R, Kroll KT, Hiratsuka K, Gupta NR, Morizane R, Lewis JA, Satlin LM. Functional maturation of kidney organoid tubules: PIEZO1-mediated Ca2+ signaling. Am J Physiol Cell Physiol. 2023 Mar 1;324(3):C757-C768. doi: 10.1152/ajpcell.00288.2022. Epub 2023 Feb 6. PMID: 36745528; PMCID: PMC10027089.

Long Name

Piezo-type mechanosensitive ion channel component 1

Entrez Gene IDs

9780 (Human); 361430 (Rat)

Alternate Names

FAM38A; KIAA0233; member A; membrane protein induced by beta-amyloid treatment; Mib; PIEZO1; piezo-type mechanosensitive ion channel component 1; Protein FAM38A