Human pIgR Antibody Summary
Lys19-Arg638
Accession # CAA51532
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human pIgR by Western Blot. Western blot shows lysates of human stomach tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human pIgR Monoclonal Antibody (Catalog # MAB2717) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for pIgR at approximately 85-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human pIgR by Simple WesternTM. Simple Western lane view shows lysates of human stomach tissue, loaded at 1 mg/mL. A specific band was detected for pIgR at approximately 110-150 kDa (as indicated) using 2.5 µg/mL of Mouse Anti-Human pIgR Monoclonal Antibody (Catalog # MAB2717). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: pIgR
The human polymeric immunoglobulin receptor (pIgR; also known as membrane secretory component) is an approximately 100 kDa type I transmembrane glycoprotein that is synthesized as a 764 amino acid (aa) precursor. It includes a signal sequence (aa 1-18), an extracellular region (aa 19-638), a transmembrane segment (aa 639-661), and a cytoplasmic domain (aa 662-764) (1-3). The extracellular region consists of five Ig-like domains and a sixth non-Ig domain that connects to the membrane region. pIgR is expressed on secretory epithelial cells of exocrine tissues. Immunoglobulin isotypes consist of two heavy (H) and two light (L) chains. For IgA and IgM, this H2L2 monomer can form larger polymers through association with a joining chain (J chain). The Fc regions of IgA and IgM have a carboxy-terminal extension called a secretory tailpiece that binds the J chain (4). pIgR functions as a carrier that transports IgA and IgM across epithelium (5). On the basolateral surface of epithelial cells, the receptor initially binds non-covalently to IgA via a docking site on the J chain. This initiates a rearrangement in which a disulfide bond forms between pIgR and an IgA heavy chain (2). The complexes are then internalized and transcytosed to the apical surface. A soluble covalent complex called secretory IgA (SIgA) is now generated by proteolytic cleavage of the sixth extracellular domain of pIgR and released into the lumen (6). This IgA-bound and proteolytically generated pIgR fragment is referred to as secretory component (SC). Notably, human pIgR transcytoses constitutively, with or without ligand, creating both bound and free, 78 kDa SC following cleavage (3). The extracellular region of pIgR shares 64%, 65%, and 70% aa sequence identity with the equivalent region of rat, mouse and porcine pIgR, respectively. The receptor component of the complex anchors the SIgA molecule to mucous (7). SIgA is a crucial component of the mucosal immune system serving to protect the large expanse of mucous membranes that form a barrier between the interior of the body and the external environment (8).
- Krajci, P. et al. (1989) Biochem. Biophys. Res. Commun. 158:783.
- Piskurich, J. et al. (1995) J. Immunol. 154:1735.
- Brandtzaeg, P. and F-E. Johansen (2001) Trends Immunol. 22:545.
- Braathen, R. et al. (2002) J. Biol. Chem. 277:42755.
- Ben-Hur, H. et al. (2004) Int. J. Mol. Med. 14:35.
- Asano, M. et al. (2004) Immunology 112:583.
- Phalipon, A. and B. Corthesy (2003) Trends Immunol. 24:55.
- Uren, T. et al. (2003) J. Immunol. 170:2531.
Product Datasheets
Citation for Human pIgR Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Streptococcus pneumoniae Interacts with pIgR expressed by the brain microvascular endothelium but does not co-localize with PAF receptor.
Authors: Iovino, Federico, Molema, Grietje, Bijlsma, Jetta J
PLoS ONE, 2014-05-19;9(5):e97914.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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