Human pIgR Antibody

Catalog #: AF2717
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2717
AF2717-SP

Detection of Human pIgR by Western Blot.

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All pIgR Antibodies

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Citations (3)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human pIgR Antibody Summary

Species Reactivity

Human

Specificity

Detects human pIgR in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse pIgR is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human pIgR (R&D Systems, Catalog # 2717-PG)
Lys19-Arg638
Accession # CAA51532

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Blockade of Receptor-ligand Interaction

In a functional ELISA, 1-5 µg/mL of this antibody will block 50% of the binding of 150 ng/mL of human IgM to immobilized Recombinant Human pIgR (Catalog # 2717-PG) coated at 2 µg/mL (100 µL/well). At 40 μg/mL, this antibody will block >90% of the binding.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human pIgR by Western Blot. Western blot shows lysates of human small intestine tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human pIgR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2717) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for pIgR at approximately 85-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: pIgR

The human polymeric immunoglobulin receptor (pIgR; also known as membrane secretory component) is a 100 kDa type I transmembrane glycoprotein that is synthesized as a 764 amino acid (aa) precursor. It includes a signal sequence (aa 1-18), an extracellular region (aa 19-638), a transmembrane segment (aa 639-661), and a cytoplasmic domain (aa 662-764) (1-3). The extracellular region consists of five Ig-like domains and a sixth non-Ig domain that connects to the membrane region. pIgR is expressed on secretory epithelial cells of exocrine tissues. Immunoglobulin isotypes consist of two heavy (H) and two light (L) chains. For IgA and IgM, this H₂L₂ monomer can form larger polymers through association with a joining chain (J chain). The Fc regions of IgA and IgM have a carboxy-terminal extension called a secretory tailpiece that binds the J chain (4). pIgR functions as a carrier that transports IgA and IgM across epithelium (5). On the basolateral surface of epithelial cells, the receptor initially binds non-covalently to IgA via a docking site on the J chain. This initiates a rearrangement in which a disulfide bond forms between pIgR and an IgA heavy chain (2). The complexes are then internalized and transcytosed to the apical surface. A soluble covalent complex called secretory IgA (SIgA) is now generated by proteolytic cleavage of the sixth extracellular domain of pIgR and released into the lumen (6). This IgA-bound and proteolytically generated pIgR fragment is referred to as secretory component (SC). Notably, human pIgR transcytoses constitutively, with or without ligand, creating both bound and free, 78 kDa SC following cleavage (3). The extracellular region of pIgR is 64%, 65%, and 70% aa identical to the equivalent region in rat, mouse and porcine, respectively. The receptor component of the complex anchors the SIgA molecule to mucous (7). SIgA is a crucial component of the mucosal immune system serving to protect the large expanse of mucous membranes that form a barrier between the interior of the body and the external environment (8).

References

Krajci, P. et al. (1989) Biochem. Biophys. Res. Commun. 158:783.
Piskurich, J. et al. (1995) J. Immunol. 154:1735.
Brandtzaeg, P. and F-E. Johansen (2001) Trends Immunol. 22:545.
Braathen, R. et al. (2002) J. Biol. Chem. 277:42755.
Ben-Hur, H. et al. (2004) Int. J. Mol. Med. 14:35.
Asano, M. et al. (2004) Immunology 112:583.
Phalipon, A. and B. Corthesy (2003) Trends Immunol. 24:55.
Uren, T. et al. (2003) J. Immunol. 170:2531.

Long Name

Polymeric Immunoglobulin Receptor

Entrez Gene IDs

5284 (Human); 18703 (Mouse)

Alternate Names

FLJ22667; hepatocellular carcinoma associated protein TB6; Hepatocellular carcinoma-associated protein TB6; MGC125361; MGC125362; pIgR; Poly-Ig receptor; polymeric immunoglobulin receptor

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Citations for Human pIgR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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