Human PKC delta Antibody

Catalog #: AF4585
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4585
AF4585-SP

Detection of Human PKCδ by Western Blot.

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All PKC delta Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PKC delta Antibody Summary

Species Reactivity

Human

Specificity

Detects human PKCδ in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human PKCδ
His549-Asp676
Accession # Q05655

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human PKCδ by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line and HT-29 human colon adenocarcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human PKCd Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4585) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PKCd at approximately 78 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PKC delta

PKCδ (protein kinase C-delta) is a 78 kDa member of the PKC subfamily, AGC Ser/Thr protein kinase family of enzymes. It is a Ca⁺⁺‑independent and phospholipid‑dependent ubiquitous enzyme that participates in cellular apoptosis. Human PKCδ is 676 amino acids (aa) in length. It contains two general regions: a phosphotyrosine and DAG-type 2 binding region (aa's 1‑90 and 159‑208, respectively), and an ATP-binding catalytic region (aa 349‑603). Functionally, inactive cytoplasmic PKCδ is first activated by Tyr64 and Tyr155 phosphorylation. This induces PKCδ entry into the nucleus, where it is cleaved into 43 kDa and 36 kDa fragments, initiating apoptosis. There are two potential alternate start sites for PKCδ at Met51 and Met74. In some cells, a poorly characterized 160 kDa form of PKCδ has been noted. Over aa 549‑676, human PKCδ is 85% aa identical to mouse PKCδ.

Long Name

Protein Kinase C delta

Entrez Gene IDs

5580 (Human)

Alternate Names

EC 2.7.11; EC 2.7.11.13; MAY1; MGC49908; nPKC-delta; PKC delta; PKCD; PKCy; PRKCD; protein kinase C delta type; protein kinase C delta VIII; protein kinase C, delta

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human PKC delta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

p63 and p73, members of the p53 gene family, transactivate PKCdelta.
Authors: Ponassi, Raffaell, Terrinoni, Alessand, Chikh, Anissa, Rufini, Alessand, Lena, Anna Mar, Sayan, Berna S, Melino, Gerry, Candi, Eleonora
Biochem Pharmacol, 2006-09-07;72(11):1417-22.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot