Human PKC zeta Antibody

Name: Human PKC zeta Antibody
Brand: R&D Systems
SKU: MAB6666
Rating: 4 (3 reviews)

Catalog #: MAB6666 3 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6666
MAB6666-SP

Detection of Human PKC zeta by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PKC zeta Antibody Summary

Species Reactivity

Human

Specificity

Detects human PKC zeta in direct ELISAs and Western blots. In direct ELISAs, less than 25% cross-reactivity with recombinant human (rh) PKC iota and rhPKC alpha and no cross-reactivity with rhPKC epsilon, eta, beta 1, beta 2, delta, gamma, nu, mu, or theta is observed. In Western blots, 100% cross-reactivity with recombinant human (rh) PKC iota and no cross-reactivity with rhPKC epsilon or eta is observed.

Source

Monoclonal Mouse IgG_2B Clone # 453513

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human PKC zeta
Ile454-Val592
Accession # Q05513

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human PKC zeta by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human PKC zeta Monoclonal Antibody (Catalog # MAB6666) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PKC zeta at approximately 78 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PKC zeta

PKC zeta (protein kinase C-zeta, EC=2.7.11.13), also called PKC2, is a 592 amino acid (aa), 79 kDa member of the atypical PKC (aPKC) subfamily of Ser/Thr protein kinases. aPKC proteins can be activated by phorbol esters, but are insensitive to calcium and diacylglycerol. PKC zeta regulates cell polarity in early development, and is necessary for cytokine-induced chemotaxis of macrophages and cancer cells, chemokine-induced adhesion of neutrophils, and polarization of Th2 cells. It is activated by PDK1 phosphorylation, binds ceramide, and translocates to the plasma membrane where it regulates adherens and tight junction formation. PKC zeta contains an OPR domain (aa 15-98), a zinc finger region (aa 130-180), and a protein kinase domain (aa 252-518). PKC zeta potential variants of 409 or 539 aa have alternate start sites at aa 184 or aa 9 (also missing aa 186-233), respectively. Within the region used as an immunogen, human PKC zeta shares 98% aa identity with mouse and rat PKC zeta.

Long Name

Protein Kinase C zeta

Entrez Gene IDs

5590 (Human); 18762 (Mouse); 25522 (Rat)

Alternate Names

EC 2.7.11; EC 2.7.11.13; nPKC-zeta; PKC zeta; PKC2; PKC2PKC-ZETA; PRKCZ; protein kinase C zeta type; protein kinase C, zeta