Human Placental Lactogen/CSH1 Antibody Summary
Val27-Phe217
Accession # P01243
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Human Placental Lactogen/CSH1 by Western Blot. Western blot shows lysates of human placenta tissue. PVDF Membrane was probed with 2 µg/mL of Human Placental Lactogen/CSH1 Monoclonal Antibody (Catalog # MAB5757) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Placental Lactogen/CSH1 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
View Larger
Placental Lactogen/CSH1 in Human Placenta. Placental Lactogen/CSH1 was detected in immersion fixed paraffin-embedded sections of human placenta using Human Placental Lactogen/CSH1 Monoclonal Antibody (Catalog # MAB5757) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in syncytiotrophoblast cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Placental Lactogen/CSH1
Human Placental Lactogen (abbreviated PL or hPL), also called chorionic somatomammotropin hormone 1 (abbreviated CSH1), is a member of the prolactin/growth hormone (PRL/GH) family (1). It is found in a cluster of growth hormones on chromosome 17 that appear to have a common ancestry. Identical 191 amino acid (aa) mature hPL proteins may be formed from one of two genes (2). PL contains a pair of C-terminal cysteines that may form either intra- or interchain disulfides. Human PL shares 98% aa identity with chimpanzee PL and >85% aa sequence identity with other human growth hormones, but only ~25% aa identity with mouse, ovine or bovine PL. PL is mainly expressed by cells in the syncytiotrophoblast layer of the placenta, which produce increasing amounts of PL as pregnancy proceeds. The major portion enters the maternal circulation, where it joins GH2 (placenta-specific GH) in replacing the functions of pituitary GH during pregnancy. A smaller amount of PL circulates in the fetus. Primate PL shows high affinity for the PRL receptor and low affinity for the GH receptor (1). Reduced stimulation of PL by angiotensin 2 correlates with intrauterine growth restriction (3). There is some evidence that mature angiogenic PL may be cleaved to form an anti-angiogenic N-terminal fragment (4). Although PL promotes pancreatic beta cell survival, it does not appear to be altered in gestational diabetes. It helps prepare mammaries for lactation, but probably does not influence lactation itself. PL may be a ligand of stabilin-1, which has been proposed to regulate PL internalization and degradation or re-expression (6).
- Handwerger, S. and M. Freemark (2000) J. Ped. Endocrinol. Metab. 13:343.
- Selby, M.J. et al. (1984) J. Biol. Chem. 259:13131.
- Szukiewicz, D. et al. (2008) Int. Immunopharmacol. 8:177.
- Struman, I. et al. (1999) Proc. Natl. Acad. Sci. USA 96:1246.
- Fujinaka, Y. et al. (2007) J. Biol. Chem. 282:30707.
- Kzhyshkowska, J. et al. (2008) J. Immunol. 180:3028.
Product Datasheets
Citation for Human Placental Lactogen/CSH1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
-
Placental lactogen is expressed but is not translated into protein in breast cancer.
Authors: Tuttle T, Hugo E, Tong W, Ben-Jonathan N
PLoS ONE, 2014-01-24;9(1):e87325.
Species: Human
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC, Western Blot
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human Placental Lactogen/CSH1 Antibody
There are currently no reviews for this product. Be the first to review Human Placental Lactogen/CSH1 Antibody and earn rewards!
Have you used Human Placental Lactogen/CSH1 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
