Human Pleiotrophin/PTN Antibody

Catalog #: MAB252R Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB252R-100
MAB252R-SP
MAB252R-500
MAB252R-01M

Pleiotrophin/PTN in PC‑3 Human Cell Line.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Pleiotrophin/PTN Antibody Summary

Species Reactivity

Human

Specificity

Detects human Pleiotrophin in direct ELISAs.

Source

Recombinant Monoclonal Rat IgG_2B Clone # 851406R

Purification

Protein A or G purified from cell culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Pleiotrophin/PTN
Gly33-Asp168
Accession # P21246

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Pleiotrophin/PTN Monoclonal Antibody (Catalog # MAB2521 or Catalog # MAB2522 ).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Pleiotrophin/PTN in PC‑3 Human Cell Line. Pleiotrophin/PTN was detected in immersion fixed PC-3 human prostate cancer cell line using Recombinant Rat Anti-Human Pleiotrophin/PTN Monoclonal Antibody (Catalog # MAB252R) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pleiotrophin/PTN

Pleiotrophin (PTN), also called heparin-binding growth-associated molecule (HB-GAM), heparin-binding neurotrophic factor (HBNF), heparin-affinity regulatory peptide (HARP), or osteoblast-specific factor (OSF-1), is an 18 kDa secreted, strongly heparin-binding, developmentally regulated cytokine (1-3). PTN and Midkine share 50% amino acid (aa) sequence identity, share some functions, and constitute a family (1-3). The second of two TSP1 domains contains the highest affinity binding site for heparin (4, 5). A 15 kDa form which lacks the C-terminus is mitogenic for glioblastoma cells, while full-length PTN is not (6). PTN is a highly conserved protein; human, mouse, rat, canine, porcine, equine and bovine PTN share 98% aa sequence identity or greater. During development, PTN is involved in development of brain, bone, and organs undergoing branching morphogenesis (3). In the adult, it is induced by PDGF and upregulated in many cancers, hematopoietic stem cells and tissues undergoing remodeling (7-10). Cell surface receptors for PTN include Syndecan-3 (which mediates neurite outgrowth) and the receptor tyrosine phosphatase PTPRB, also called RPTP beta / zeta (3, 11-13). Heparin binding is necessary for engaging these receptors (7, 8). PTN causes PTPRB dimerization and inactivates its phosphatase activity, which allows increased tyrosine phosphorylation of its substrates (12-14). One such substrate is the WNT pathway molecule beta -catenin, allowing crosstalk of PTN with WNTs (12). PTN activation of the receptor ALK (anaplastic lymphoma kinase) is indirect through PTPRB, and mediates mitogenic, transforming and angiogenic activities of PTN (2, 5, 6, 13). Increased expression of PTN is correlated with neuronal development or stresses such as brain ischemia and Parkinson’s disease (2, 3, 7, 8). Both PTN and Midkine have demonstrated bactericidal activity, but only in the absence of heparin (15).

References

Naito, A. et al. (1992) Biochem. Biophys. Res. Commun. 183:701.
Perez-Pinera, P. et al. (2008) Curr. Opin. Hematol. 15:210.
Weng, T. and L. Liu (2010) Respir. Res. 11:80.
Raulo, E. et al. (2005) J. Biol. Chem. 280:41576.
Hamma-Kourbali, Y. et al. (2008) J. Cell. Physiol. 214:250.
Lu, K.V. et al. (2005) J. Biol. Chem. 280:16953.
Yeh, H-J. et al. (1998) J. Neurosci. 18:3699.
Marchionini, D.M. et al. (2007) Brain Res. 1147:77.
Chang, Y. et al. (2007) Proc. Natl. Acad. Sci. USA 104:10888.
Himburg, H.A. et al. (2010) Nat. Med. 16:475.
Kinnunen, T. et al. (1996) J. Biol. Chem. 271:2243.
Meng, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:2603.
Perez-Pinera, P. et al. (2007) J. Biol. Chem. 282:28683.
Fukada, M. et al. (2006) FEBS Lett. 580:4051.
Svensson, S.L. et al. (2010) J. Biol. Chem. 285:16105.

Entrez Gene IDs

5764 (Human)

Alternate Names

HARP; HBBM; HB-GAM; HBGF8; HBNF; HBNF1; HBNF-1; Heparin-binding brain mitogen; HNGF-8; NEGF1HBGF-8; neurite growth-promoting factor 1; neurite growth-promoting factor1); OSF-1; Osteoblast-specific factor 1; Pleiotrophin; PTN