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Human Plexin B2 Antibody

Catalog #: MAB5329
2 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB5329
MAB5329-SP
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (1)
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Plexin B2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Plexin B2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Plexin A4, B1, B3, C1, D1, or recombinant mouse Plexin A1, A2, or A3 is observed.
Source
Monoclonal Mouse IgG2A Clone # 537237
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Plexin B2
Leu20-Arg1160
Accession # O15031
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Plexin B2 under non-reducing conditions only

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Plexin B2

Plexin B2 is a 240 kDa member of the B subfamily, plexin family of semaphorin receptors. It is expressed by cerebellar granule cells, neuroepithelium and chondrocytes, and serves as a receptor for Semaphorin 4C. Mature human Plexin B2 is 1819 amino acid (aa) in length. It is a type I transmembrane (TM) glycoprotein that contains an 1178 aa extracellular domain (ECD) plus a 620 aa cytoplasmic region. The ECD contains one semaphorin domain (aa 20‑466) and three IPT repeats (aa 803‑1092). The ECD may be cleaved into two subunits, a 170 kDa alpha -chain (aa 20‑1164) and an 80 kDa TM beta ‑chain that remain noncovalently‑linked. Multiple splice variants may exist. One shows an alternate start site at Met1369, while a second shows a deletion of aa 67‑638. Over aa 20‑1160, human Plexin B2 is 82% aa identical to mouse Plexin B2.

Entrez Gene IDs
23654 (Human); 140570 (Mouse); 315217 (Rat)
Alternate Names
MM1; MM1KIAA0315dJ402G11.3; Nbla00445; PLEXB2; Plexin B2; plexin-B2; PLXNB2

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Citations for Human Plexin B2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Semaphorin 4D is upregulated in neurons of diseased brains and triggers astrocyte reactivity
    Authors: EE Evans, V Mishra, C Mallow, EM Gersz, L Balch, A Howell, C Reilly, ES Smith, TL Fisher, M Zauderer
    Journal of Neuroinflammation, 2022-08-06;19(1):200.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  2. Semaphorin 4A exerts a proangiogenic effect by enhancing vascular endothelial growth factor-A expression in macrophages.
    Authors: Meda C, Molla F, De Pizzol M
    J. Immunol., 2012-03-21;188(8):4081-92.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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Reviews for Human Plexin B2 Antibody

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Human Plexin B2 Antibody
By Anonymous on 07/27/2016
Application: WB Sample Tested: B cells Species: Human