Human Plexin B2 Biotinylated Antibody

Catalog #: BAF5329 Datasheet / COA / SDS

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BAF5329

All Plexin B2 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Plexin B2 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Plexin B2 in Western blots. In Western blots, less than 1% cross‑reactivity with recombinant human (rh) Plexin A4, rhPlexin B1, rhPlexin B3, rhPlexin C1, and rhPlexin D1 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Plexin B2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Plexin B2

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Plexin B2

Plexin B2 is a 240 kDa member of the B subfamily, Plexin family of semaphorin receptors. It is expressed by cerebellar granule cells, neuroepithelium and chondrocytes and serves as a receptor for Sema4C. Mature human Plexin B2 is 1819 amino acid (aa) in length. It is a type I transmembrane (TM) glycoprotein that contains an 1178 aa extracellular domain (ECD) plus a 620 aa cytoplasmic region. The ECD contains one semaphorin domain (aa 20‑466) and three IPT repeats (aa 803‑1092). The ECD may be cleaved into two subunits, a 170 kDa alpha ‑chain (aa 20‑1164) and an 80 kDa TM beta -chain that remain noncovalently-linked. Multiple splice variants may exist. One shows an alternate start site at Met1369, while a second shows a deletion of aa 67‑638. Over aa 20‑1160, human Plexin B2 is 82% aa identical to mouse Plexin B2.

Entrez Gene IDs

23654 (Human); 140570 (Mouse); 315217 (Rat)

Alternate Names

MM1; MM1KIAA0315dJ402G11.3; Nbla00445; PLEXB2; Plexin B2; plexin-B2; PLXNB2