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Human PlGF Antibody

Catalog #: AF-264-PB
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF-264-PB
AF-264-SP
Detection of Human PlGF by Western Blot.
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Product Details
Citations (6)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PlGF Antibody Summary

Species Reactivity
Human
Specificity
Detects human PlGF (but not VEGF) in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human PlGF
Ala21-Arg149
Accession # CAA38698
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human PlGF antibody by Western Blot. View Larger

Detection of Human PlGF by Western Blot. Western blot shows lysates of CHO Chinese hamster ovary cell line either mock transfected or transfected with full length human PlGF. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human PlGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-264-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PlGF at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PlGF

Placenta Growth Factor (PlGF) is a member of the Vascular Endothelial Growth Factor (VEGF) family of growth factors. PlGF and VEGF share primary structural as well as limited amino acid sequence homology with the A and B chains of PDGF. All eight cysteine residues involved in intra- and inter-chain disulfides are conserved among these growth factors. In their PDGF-like regions, VEGF and PlGF also share approximately 53% amino acid sequence similarity. The gene for PlGF has been mapped to chromosome 14. As a result of alternative splicing, at least two PlGF mRNAs encoding monomeric PlGF precursors containing 149 and 170 amino acid residues have been described. The expression of PlGF is not widespread, but has been detected in human umbilical vein endothelial cells, placenta, choriocarcinoma cell lines, and in renal cell carcinoma associated with angiogenesis. The PlGF proteins bind with high-affinity to Flt-1, but not to Flk-1/KDR.

Long Name
Placenta Growth Factor
Entrez Gene IDs
5228 (Human); 18654 (Mouse)
Alternate Names
D12S1900; PGF; PGFL; placenta growth factor; placental growth factor; placental growth factor, vascular endothelial growth factor-related protein; PlGF; PlGF-2; PLGFplacental growth factor-like; SHGC-10760

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Citations for Human PlGF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Role of IL-36 Cytokines in the Regulation of Angiogenesis Potential of Trophoblast Cells
    Authors: JM Murrieta-C, RN Gutiérrez-, HM El-Shorafa, T Groten, S Rodríguez-, ME Cancino-Di, JC Cancino-Di, RR Favaro, UR Markert, DM Morales-Pr
    International Journal of Molecular Sciences, 2020-12-30;22(1):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Discovery of pan-VEGF inhibitory peptides directed to the extracellular ligand-binding domains of the VEGF receptors
    Authors: Jussara S. Michaloski, Alexandre R. Redondo, Leila S. Magalhães, Caio C. Cambui, Ricardo J. Giordano
    Science Advances
  3. Placental growth factor neutralising antibodies give limited anti-angiogenic effects in an in vitro organotypic angiogenesis model.
    Authors: Brave SR, Eberlein C, Shibuya M, Wedge SR, Barry ST
    Angiogenesis, 2010-10-16;13(4):337-47.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. VEGF-A and VEGF-F evoke distinct changes in vascular ultrastructure.
    Authors: Matsunaga Y, Yamazaki Y, Suzuki H, Morita T
    Biochem. Biophys. Res. Commun., 2009-01-04;379(4):872-5.
    Species: Guinea Pig
    Sample Types: Whole Tissue
    Applications: IHC
  5. Malignant mesothelioma growth inhibition by agents that target the VEGF and VEGF-C autocrine loops.
    Authors: Masood R, Kundra A, Zhu S, Xia G, Scalia P, Smith DL, Gill PS
    Int. J. Cancer, 2003-05-01;104(5):603-10.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Vascular endothelial growth factor ligands and receptors that regulate human cytotrophoblast survival are dysregulated in severe preeclampsia and hemolysis, elevated liver enzymes, and low platelets syndrome.
    Authors: Zhou Y, 2019, McMaster M, Woo K, Janatpour M, Perry J, Karpanen T, Alitalo K, Damsky C, Fisher SJ
    2002-04-01;160(4):1405-23.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC

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