Human PLK3 Antibody

Catalog #: AF4197 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4197
AF4197-SP

Detection of Human PLK3 by Western Blot.

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Human PLK3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human PLK3 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human PLK3
Pro320-Ala520
Accession # Q9H4B4.2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human PLK3 by Western Blot. Western blot shows nuclear extracts of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human PLK3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4197) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PLK3 at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PLK3

Polo-like kinases are critical regulators of cell cycle progression, cytokinesis, mitosis, and the genotoxic stress response. Four distinct members have been identified to date in mammalian cells: PLK1, PLK2, PLK3, and PLK4. PLK3 (polo-like kinase 3) plays an important role in cell cycle regulation by modulating microtubule dynamics and centrosomal function. Although protein levels are unchanged throughout the cell cycle, kinase activity peaks during late S and G2 phases. Many key cell cycle regulators are phosphorylated by PLK3 including p53 on Ser20, Cdc25C on Ser216, and the major subunit of DNA polymerase δ, p125 on Ser60.

Long Name

Polo-like Kinase 3

Entrez Gene IDs

1263 (Human); 12795 (Mouse); 58936 (Rat)

Alternate Names

CNK; CNKpolo-like kinase 3 (Drosophila); cytokine-inducible kinase; Cytokine-inducible serine/threonine-protein kinase; EC 2.7.11; FNK; FNKEC 2.7.11.21; PLK3; polo-like kinase 3PLK-3; PRK; PRKFGF-inducible kinase; Proliferation-related kinase; serine/threonine-protein kinase PLK3