Human PLTP Antibody

Catalog #: AF5109 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5109
AF5109-SP

Detection of human PLTP by Western Blot.

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Human PLTP Antibody Summary

Species Reactivity

Human

Specificity

Detects human PLTP in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse PLTP is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human PLTP isoform 1
Glu18-Val493 (Glu18Val)
Accession # P55058

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Knockout Validated

PLTP is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in PLTP knockout HeLa cell line.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of human PLTP by Western Blot. Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human PLTP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5109) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PLTP at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Knockout Validated

Western Blot Shows Human PLTP Antibody Specificity by Using Knockout Cell Line.

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Western Blot Shows Human PLTP Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and PLTP knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human PLTP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5109) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PLTP at approximately 75 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PLTP

PLTP (phospholipid transfer protein) is a 75-81 kDa, secreted glycoprotein member of the BPI/LBP family of proteins. It is expressed by multiple cell types, circulates bound to HDL, and mediates the transfer of phospholipids and cholesterol from apoB-containing lipoproteins to HDL. Mature human PLTP is 476 amino acids (aa) in length. It contains an N-terminal lipid transfer domain and a C-terminal HDL-binding domain; there is one essential intrachain disulfide bond (Cys146-Cys185). There are four potential PLTP splice variants. Two show alternate start sites at Met21 and Met89. Two others demonstrate aa substitutions; one shows a one aa (Lys) substitution for aa 110-162, and a second shows a four aa substitution for the N-terminal 35 amino acids. Over aa 18-493, human PLTP shares 92% and 83% aa identity with porcine and mouse PLTP, respectively.

Long Name

Phospholipid Transfer Protein

Entrez Gene IDs

5360 (Human); 18830 (Mouse); 296371 (Rat)

Alternate Names

HDLCQ9; Lipid transfer protein II; phospholipid transfer protein; PLTP