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Human PNMT Antibody

Catalog #: AF7854
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF7854
AF7854-SP
Detection of Human PNMT by Western Blot.
2 Images
Product Details
Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PNMT Antibody Summary

Species Reactivity
Human
Specificity
Detects human PNMT in direct ELISAs and Western blots. In direct ELISAs, less than 4% cross-reactivity with recombinant human (rh) INMT and rhNNMT is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human PNMT
Ser2-Leu282
Accession # P11086
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human PNMT antibody by Western Blot. View Larger

Detection of Human PNMT by Western Blot. Western blot shows lysates of human adrenal gland tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human PNMT Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7854) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PNMT at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry PNMT antibody in K562 Human Cell Line by Immunocytochemistry (ICC). View Larger

PNMT in K562 Human Cell Line. PNMT was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Sheep Anti-Human PNMT Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7854) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PNMT

PNMT (Phenylethanolamine N-MethylTransferase; also PENT and Noradrenaline N-methyltransferase) is a monomeric, 30-34 kDa cytoplasmic member of the NNMT/PNMT/TEMT family of enzymes. It has limited expression, being found in Leydig, skeletal muscle and B cells, adrenal chromaffin cells, select adrenergic neurons of the vagus nerve, periventricular grey and reticular formation, and specialized adrenalin-containing cells contained within the heart. PNMT converts noradrenalin into adrenalin by transferring a methyl group from AdoMet to the terminal amine group of noradrenalin. Human PNMT is 282 amino acids (aa) in length and contains an active enzyme site between aa 14-279. Full length human PNMT1 shares 81% aa sequence identity with mouse PNMT.

Long Name
Phenylethanolamine N-Methyltransferase
Entrez Gene IDs
5409 (Human); 18948 (Mouse)
Alternate Names
EC 2.1.1; EC 2.1.1.28; Noradrenaline N-methyltransferase; PENT; PENTMGC34570; phenylethanolamine N-methylase; phenylethanolamine N-methyltransferase; PNMT; PNMTase

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Citation for Human PNMT Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Noncanonical genomic imprinting in the monoamine system determines naturalistic foraging and brain-adrenal axis functions
    Authors: PJ Bonthuis, S Steinwand, CN Stacher Hö, J Emery, WC Huang, S Kravitz, E Ferris, C Gregg
    Cell Reports, 2022-03-08;38(10):110500.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC

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