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Human Podocalyxin Antibody

Catalog #: MAB1658
22 Citations 6 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1658
MAB1658-SP
Detection of Podocalyxin in BG01V Human Cells by Flow Cytometry.
3 Images
Product Details
Citations (22)
FAQs
Supplemental Products
Reviews (6)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Podocalyxin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Podocalyxin in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse Podocalyxin or recombinant human Endoglycan is observed.
Source
Monoclonal Mouse IgG2A Clone # 222328
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Podocalyxin
Ser23-Arg427
Accession # AAB61574
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Podocalyxin
Flow Cytometry
0.25 µg/106 cells
- BG01V human embryonic stem cells
- Induced pluripotent stem cells (iPSCs)
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Podocalyxin antibody in BG01V Human Cells antibody by Flow Cytometry. View Larger

Detection of Podocalyxin in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells was stained with Mouse Anti-Human Podocalyxin Monoclonal Antibody (Catalog # MAB1658, filled histogram) or isotype control antibody (MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry Podocalyxin antibody in BG01V Human Stem Cells by Immunocytochemistry (ICC). View Larger

Podocalyxin in BG01V Human Stem Cells. Podocalyxin was detected in immersion fixed BG01V human embryonic stem cells using 10 µg/mL Mouse Anti-Human Podocalyxin Monoclonal Antibody (Catalog # MAB1658) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Flow Cytometry View Larger

Detection of Podocalyxin in Induced pluripotent stem cells (iPSCs) by Flow Cytometry Induced pluripotent stem cells (iPSCs) were stained with Mouse Anti-Human Podocalyxin Monoclonal Antibody (Catalog # MAB1658, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram) followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Podocalyxin

Podocalyxin, also known as Podocalyxin-like protein-1 (PCLP1 or PODXL), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology (1). Podocalyxin is a major sialoprotein in the podocytes of the kidney glomerulus and is also expressed by both endothelium and multipotent hematopoietic progenitors (2). It has been identified as a novel cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells.

References
  1. Li, J. et al. (2001) DNA Seq. 12:407.
  2. Hara, T. et al. (1999) Immunity 11:567.
Entrez Gene IDs
5420 (Human); 27205 (Mouse)
Alternate Names
GCTM; Gp200MGC138240; PCLP; PCLP1; PCLP-1; PCLPGCTM-2 antigen; PCpodocalyxin; POD XL; Podocalyxin; Podocalyxin-like protein 1; podocalyxin-like; PODXL

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Citations for Human Podocalyxin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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  1. A single cell characterisation of human embryogenesis identifies pluripotency transitions and putative anterior hypoblast centre
    Authors: Matteo A. Molè, Tim H. H. Coorens, Marta N. Shahbazi, Antonia Weberling, Bailey A. T. Weatherbee, Carlos W. Gantner et al.
    Nature Communications
  2. Podocalyxin Expressed in Antigen Presenting Cells Promotes Interaction With T Cells and Alters Centrosome Translocation to the Contact Site
    Authors: Laura Amo, Javier Díez-García, Estíbaliz Tamayo-Orbegozo, Natalia Maruri, Susana Larrucea
    Frontiers in Immunology
  3. Modeling kidney development, disease, and plasticity with clonal expandable nephron progenitor cells and nephron organoids
    Authors: Huang B, Zeng Z, Li H et al.
    bioRxiv
  4. Distinct pathways drive anterior hypoblast specification in the implanting human embryo
    Authors: Weatherbee, BAT;Weberling, A;Gantner, CW;Iwamoto-Stohl, LK;Barnikel, Z;Barrie, A;Campbell, A;Cunningham, P;Drezet, C;Efstathiou, P;Fishel, S;Vindel, SG;Lockwood, M;Oakley, R;Pretty, C;Chowdhury, N;Richardson, L;Mania, A;Weavers, L;Christie, L;Elder, K;Snell, P;Zernicka-Goetz, M;
    Nature cell biology
    Species: Human, Mouse
    Sample Types: Embryo
    Applications: Immunohistochemistry
  5. PCDH12 loss results in premature neuronal differentiation and impeded migration in a cortical organoid model
    Authors: Rakotomamonjy, J;Rylaarsdam, L;Fares-Taie, L;McDermott, S;Davies, D;Yang, G;Fagbemi, F;Epstein, M;Fairbanks-Santana, M;Rozet, JM;Guemez-Gamboa, A;
    Cell reports
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. Modeling kidney development, disease, and plasticity with clonal expandable nephron progenitor cells and nephron organoids
    Authors: Huang B, Zeng Z, Li H et al.
    bioRxiv
  7. Genome-wide screening in human kidney organoids identifies developmental and disease-related aspects of nephrogenesis
    Authors: R Ungricht, L Guibbal, MC Lasbennes, V Orsini, M Beibel, A Waldt, R Cuttat, W Carbone, A Basler, G Roma, F Nigsch, JS Tchorz, D Hoepfner, PS Hoppe
    Cell Stem Cell, 2021-11-29;0(0):.
    Species: Human
    Sample Types: Organoid
    Applications: IHC
  8. Comparative evaluation of isogenic mesodermal and ectomesodermal chondrocytes from human iPSCs for cartilage regeneration
    Authors: MS Lee, MJ Stebbins, H Jiao, HC Huang, EM Leiferman, BE Walczak, SP Palecek, EV Shusta, WJ Li
    Science Advances, 2021-05-19;7(21):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Naive stem cell blastocyst model captures human embryo lineage segregation
    Authors: A Yanagida, D Spindlow, J Nichols, A Dattani, A Smith, G Guo
    Cell Stem Cell, 2021-05-05;28(6):1016-1022.e4.
    Species: Human
    Sample Types: Embryo
    Applications: ICC
  10. Identification of the SHREK Family of Proteins as Broad-Spectrum Host Antiviral Factors
    Authors: D Dabbagh, S He, B Hetrick, L Chilin, A Andalibi, Y Wu
    Viruses, 2021-05-04;13(5):.
    Species: Virus
    Sample Types: Viral Particles
    Applications: Immunoprecipitation
  11. Identification of the SHREK family of proteins as broad-spectrum host antiviral factors
    Authors: D Dabbagh, S He, B Hetrick, L Chilin, A Andalibi, Y Wu
    bioRxiv : the preprint server for biology, 2021-02-03;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ELISA Capture, ICC
  12. Developmental potential of aneuploid human embryos cultured beyond implantation
    Authors: MN Shahbazi, T Wang, X Tao, BAT Weatherbee, L Sun, Y Zhan, L Keller, GD Smith, A Pellicer, RT Scott, E Seli, M Zernicka-G
    Nat Commun, 2020-08-10;11(1):3987.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  13. Podocalyxin promotes proliferation and survival in mature B-cell non-Hodgkin lymphoma cells
    Authors: E Tamayo-Orb, L Amo, M Riñón, N Nieto, E Amutio, N Maruri, M Solaun, A Arrieta, S Larrucea
    Oncotarget, 2017-09-27;8(59):99722-99739.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Self-organization of the human embryo in the absence of maternal tissues
    Nat. Cell Biol., 2016-05-04;18(6):700-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  15. Podocalyxin-like protein is expressed in glioblastoma multiforme stem-like cells and is associated with poor outcome.
    Authors: Binder Z, Siu I, Eberhart C, ap Rhys C, Bai R, Staedtke V, Zhang H, Smoll N, Piantadosi S, Piccirillo S, Dimeco F, Weingart J, Vescovi A, Olivi A, Riggins G, Gallia G
    PLoS ONE, 2013-10-16;8(10):e75945.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  16. Characterization of renal progenitors committed toward tubular lineage and their regenerative potential in renal tubular injury.
    Authors: Angelotti ML, Ronconi E, Ballerini L, Peired A, Mazzinghi B, Sagrinati C, Parente E, Gacci M, Carini M, Rotondi M, Fogo AB, Lazzeri E, Lasagni L, Romagnani P
    Stem Cells, 2012-08-01;30(8):1714-25.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Notch activation differentially regulates renal progenitors proliferation and differentiation toward the podocyte lineage in glomerular disorders.
    Authors: Lasagni L, Ballerini L, Angelotti ML
    Stem Cells, 2010-09-01;28(9):1674-85.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  18. Regeneration of glomerular podocytes by human renal progenitors.
    Authors: Ronconi E, Sagrinati C, Angelotti ML
    J. Am. Soc. Nephrol., 2008-12-17;20(2):322-32.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Selection
  19. Human Embryonic Stem Cells Express Elevated Levels of Multiple Pro-Apoptotic BCL-2 Family Members
    Authors: David T. Madden, Diana Davila-Kruger, Simon Melov, Dale E. Bredesen
    PLoS ONE
  20. An apicosome initiates self-organizing morphogenesis of human pluripotent stem cells
    Authors: Kenichiro Taniguchi, Yue Shao, Ryan F. Townshend, Chari L. Cortez, Clair E. Harris, Sasha Meshinchi et al.
    Journal of Cell Biology
  21. Effect of Cell Spreading on Rosette Formation by Human Pluripotent Stem Cell-Derived Neural Progenitor Cells
    Authors: Ryan F. Townshend, Yue Shao, Sicong Wang, Chari L. Cortez, Sajedeh Nasr Esfahani, Jason R. Spence et al.
    Frontiers in Cell and Developmental Biology

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Reviews for Human Podocalyxin Antibody

Average Rating: 4.5 (Based on 6 Reviews)

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Human Podocalyxin Antibody
By Anonymous on 09/06/2021
Application: IHC Sample Tested: Kidney tissue Species: Human
Immunohistochemistry Human Podocalyxin Antibody MAB1658
Human Podocalyxin Antibody
By Anonymous on 01/14/2021
Application: Microarrays Sample Tested: EDTA Plasma Species: Human
Other Human Podocalyxin Antibody MAB1658
Human Podocalyxin Antibody
By Anonymous on 12/01/2020
Application: IHC Sample Tested: MAN13 hESC derived kidney organoid section Species: Human

Used at 1:200 dilution

Immunohistochemistry Human Podocalyxin Antibody MAB1658
Human Podocalyxin Antibody
By Anonymous on 11/20/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human
Human Podocalyxin Antibody
By Anonymous on 11/08/2018
Application: ELISA Sample Tested: Serum and Plasma Species: Human and Mouse
Human Podocalyxin Antibody
By Anonymous on 11/07/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human