Human Podocalyxin Antibody

Detection of Podocalyxin in MDA-MB-231 Human Cell Line by Flow Cytometry. MDA-MB-231 (orange) or MG-63 (blue) human cell lines were stained with Mouse Anti-Human Podocalyxin Monoclonal Antibody (Catalog # MAB1658, filled histograms) or isotype control antibody (MAB003, open histograms), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). View our protocol for Staining Membrane-associated Proteins.

Podocalyxin in BG01V Human Stem Cells. Podocalyxin was detected in immersion fixed BG01V human embryonic stem cells using 10 µg/mL Mouse Anti-Human Podocalyxin Monoclonal Antibody (Catalog # MAB1658) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Podocalyxin in Human Kidney. Podocalyxin was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human Podocalyxin Monoclonal Antibody (Catalog # MAB1658) at 0.05 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to glomerular epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Podocalyxin by Immunocytochemistry/ Immunofluorescence Live-tracking of NPC rosette morphogenesis. (A–C) Live imaging of 1196a hiPSC expressing Lifeact-GFP (A) or PODXL-GFP (C) for 12 h after colonies were allowed to reattach for 2 h. (B) Confocal images of PODXL-GFP NPC rosettes stained with anti-PODXL. GFP localization mirrors endogenous PODXL localization. Note that, while a uniform prominent GFP signal is initially seen throughout the apical surface of the recently plated colony, at later time points, GFP-enriched regions are concentrated at the constricted centers of the rosettes (lumen). It is likely that, as the colony expands rapidly, the apical surface area of non-rosette cells dramatically increases, resulting in reduced GFP signal intensity per unit area. Scales as indicated. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33178701), licensed under a CC-BY license. Not internally tested by R&D Systems.