Human Podoplanin APC-conjugated Antibody Summary
Glu21-Lys123
Accession # Q86YL7
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Podoplanin in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Sheep Anti-Human Podoplanin APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB3670A, filled histogram) or isotype control antibody (Catalog # IC016A, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Detection of Podoplanin in U87MG Human Cell Line by Flow Cytometry. U87MG human glioblastoma cell line was stained with Sheep Anti-Human Podoplanin APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB3670A, filled histogram) or isotype control antibody (IC016A, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
Background: Podoplanin
Podoplanin, also known as glycoprotein 36 (gp36), PA2.26 antigen, T1-alpha (T1A), and aggrus, is a 36 kDa type I transmembrane sialoglycoprotein and member of the Podoplanin family (1‑2). Podoplanin has three potential splice variants, the longest of which is represented by a 238 amino acid (aa) precursor (NP_006465). It contains an undefined signal sequence, a 22 aa transmembrane segment (aa 207‑228) and a short cytoplasmic tail (aa 229‑238). The ECD contains abundant Ser/Thr residues that could serve as potential O-linked glycosolation sites. The cytoplasmic tail contains putative sites for protein kinase C phosphorylation (2‑3). There are two potential alternate start sites at Met 77 (Swiss Prot #: Q86YL7) and Met 119 (EAW51692) that generate short forms. The 162 aa short form Podoplanin precursor shares 47% aa identity with mouse Podoplanin. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells (4‑5), and numerous tumors, including colorectal tumors (3), squamous cell carcinomas (4, 6), testicular seminoma (7), and brain tumors (8‑10). One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver (1). Podoplanin is the ligand for C-type lectin-like receptor 2 (CLEC-2) (2). Their association is dependent on sialic acid on O-glycans of Podoplanin (2). Through its association with CLEC-2, Podoplanin induces platelet aggregation and tumor metastasis (2). Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth (2).
- Zimmer, G. et al. (1999) Biochem. J. 341:277.
- Katsue-Inoue, K. et al. (2007) J. Biol. Chem. 282:25993.
- Kato, Y. et al. (2003) J. Biol. Chem. 278:51599.
- Schacht, V. et al. (2005) Am. J. Pathol. 166:913.
- Breiteneder-Geleff, S. et al. (1997) Am. J. Pathol. 151:1141.
- Kato, Y. et al. (2005) Tumour Biol. 26:195.
- Kato, Y. et al. (2004) Oncogene 23:8552.
- Mishima, K. et al. (2006) Acta Neuropathol. 111:563.
- Mishima, K. et al. (2006) Acta Neuropathol. 111:483.
- Kato, Y. et al. (2006) Biochem. Biophys. Res. Commun. 349:1301.
Product Datasheets
Citations for Human Podoplanin APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Co-culture with podoplanin+ cells protects leukemic blast cells with leukemia-associated antigens in the tumor microenvironment
Authors: JI YOON LEE, A-REUM HAN, SUNG-EUN LEE, WOO-SUNG MIN, HEE-JE KIM
Molecular Medicine Reports
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Human Lymph Node-Derived Fibroblastic and Double-Negative Reticular Cells Alter Their Chemokines and Cytokines Expression Profile Following Inflammatory Stimuli
Authors: P Severino, DT Palomino, H Alvarenga, CB Almeida, DC Pasqualim, A Cury, PR Salvalaggi, AL De Vasconc, MC Andrade, T Aloia, S Bromberg, LV Rizzo, FA Rocha, LC Marti
Front Immunol, 2017-02-14;8(0):141.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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