Human Polypeptide GalNAc Transferase 4/GALNT4 Antibody

Catalog #: AF7528 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7528
AF7528-SP

Detection of Human and Mouse Polypeptide GalNac Transferase 4/GALNT4 by Western Blot.

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All Polypeptide GalNAc Transferase 4/GALNT4 Antibodies

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Human Polypeptide GalNAc Transferase 4/GALNT4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Polypeptide GalNac Transferase 4/GALNT4 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) GALNT1 and rhGALNT3 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Polypeptide GalNac Transferase 4/GALNT4
Ala36-Lys578
Accession # Q8N4A0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse Polypeptide GalNac Transferase 4/GALNT4 by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and mouse liver tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Polypeptide GalNac Transferase 4/GALNT4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7528) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Polypeptide GalNac Transferase 4/GALNT4 at approximately 66 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Polypeptide GalNAc Transferase 4/GALNT4

GALNT4 (Polypeptide N‑acetylgalactosaminyltransferase 4; also Polypeptide GalNAc transferase 4 and UDP-GalNAc:polypeptide Nacetylgalactosaminyltransferase 4) is a 66 kDa member of the GalNAC transferase subfamily, glycosyltransferase 4 family of enzymes. It is widely expressed, highly expressed in mucous cells. GALNT4 is found in the Golgi apparatus, and catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Human GALNT4 is a 578 amino acid (aa) type II transmembrane protein. It contains a twelve aa N-terminal cytoplasmic region and a 543 aa extracellular lumenal domain (aa 36-578). There is a potential 45 kDa splice form variant. Over aa 36-578, human GALNT4 shares 92% aa sequence identity with mouse GALNT4.

Long Name

UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 4

Entrez Gene IDs

8693 (Human); 14426 (Mouse); 500826 (Rat)

Alternate Names

EC 2.4.1.41; FLJ93314; GALNACT4; GalNAc-T4; GALNT4; Polypeptide GalNAc transferase 4; polypeptide N-acetylgalactosaminyltransferase 4; pp-GaNTase 4; Protein-UDP acetylgalactosaminyltransferase 4; UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 4; UDP-N-acetyl-alpha-D-galactosamine:polypeptideN-acetylgalactosaminyltransferase 4 (GalNAc-T4)