Home / PON2 / Human PON2 Antibody

Human PON2 Antibody

Catalog #: AF4344
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4344
AF4344-SP
Detection of Human PON2 by Western Blot.
1 Image
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PON2 Antibody Summary

Species Reactivity
Human
Specificity
Detects endogenous human PON2 in Western blots. In Western blots, this antibody shows no cross-reactivity with recombinant human PON1 or PON3.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human PON2
Ala30-Leu354
Accession # Q15165
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human PON2 antibody by Western Blot. View Larger

Detection of Human PON2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human PON2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4344) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PON2 at approximately 42-45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PON2

The paraoxonase (PON) gene family of antioxidant enzymes includes three known members located adjacent to each other on chromosome 7. Paraoxonase/arylesterase 2 (PON2) is a 354 amino acid, 39 kDa protein that is widely expressed in a variety of tissues and may act as a cellular antioxidant, protecting cells from oxidative stress. PON2 has arylesterase and aryldialkylphosphatase activity (EC 3.1.1.2 and EC 3.1.8.1) and can hydrolyze a number of organophosphate substrates and aromatic carboxylic acid esters. PON2 is membrane-bound and has several potential glycosylation sites. Sequence polymorphisms in this gene may be associated with coronary heart disease and a number of phenotypes related to diabetes. PON2 is not associated with HDL but can prevent LDL lipid peroxidation and reverse the oxidation of mildly oxidized LDL. Alternatively spliced transcript variants encoding different isoforms have been described.

Long Name
Paraoxonase 2
Entrez Gene IDs
5445 (Human); 330260 (Mouse); 296851 (Rat)
Alternate Names
A-esterase 2; Aromatic esterase 2; EC 3.1.1.2; EC 3.1.1.81; paraoxonase 2; paraoxonase nirs; PON 2; PON2; Serum Aryldialkylphosphatase 2; serum paraoxonase/arylesterase 2

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human PON2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. Variation among intact tissue samples reveals the core transcriptional features of human CNS cell classes
    Authors: Kevin W. Kelley, Hiromi Nakao-Inoue, Anna V. Molofsky, Michael C. Oldham
    Nature Neuroscience
  2. Paraoxonase 2 overexpression inhibits tumor development in a mouse model of ovarian cancer
    Authors: A Devarajan, F Su, V Grijalva, M Yalamanchi, A Yalamanchi, F Gao, H Trost, J Nwokedi, G Farias-Eis, R Farias-Eis, AM Fogelman, ST Reddy
    Cell Death Dis, 2018-03-12;9(3):392.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human PON2 Antibody

There are currently no reviews for this product. Be the first to review Human PON2 Antibody and earn rewards!

Have you used Human PON2 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review