Human POT1 Antibody Summary
Met159-Pro316
Accession # Q9NUX5
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human POT1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HL-60 human acute promyelocytic leukemia cell line. PVDF Membrane was probed with 2 µg/mL of Human POT1 Monoclonal Antibody (Catalog # MAB5299) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for POT1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: POT1
POT1 (Protection of telomeres protein 1; also POTE1) is a nuclear, 71 kDa member of the telombin family of proteins. It is ubiquitously expressed, and binds single stranded DNA, TRF2, and TPP1 at the end of chromosomes. This stabilizes chromosome ends and protects against unwarranted activation of DNA damage programs. Human POT1 is 634 amino acids (aa) in length. It contains two N-terminal DNA binding OB regions (aa 1-300) and a C-terminal TPP-1 dimerization domain (aa 301-634). There are multiple isoform variants. There is a potential alternate start site at Met132, plus a three aa substitution for aa 43-634, a 24 aa substitution for aa 317-634, a three aa substitution for aa 457-634, and a 16 aa substitution for aa 503-634. Over aa 159-316, human POT1 shares 71% aa identity with mouse POT1.
Product Datasheets
Citation for Human POT1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Differential Regulation of Telomeric Complex by BCR-ABL1 Kinase in Human Cellular Models of Chronic Myeloid Leukemia-From Single Cell Analysis to Next-Generation Sequencing
Authors: A Deregowska, M Pepek, K Pruszczyk, MM Machnicki, M Wnuk, T Stoklosa
Genes (Basel), 2020-09-29;11(10):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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