Human POU2F3 Antibody

Catalog #: MAB11625 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11625-100
MAB11625-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human POU2F3 Antibody Summary

Species Reactivity

Human

Specificity

Detects a synthetic peptide specific for human POU2F3 around amino acid 100 in Direct ELISA.

Source

Monoclonal Mouse IgG_2A Clone # 1091214

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Synthetic Peptide
Accession # Q9UKI9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

3-25 µg/mL

Immersion fixed paraffin-embedded sections of human melanoma

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Detection of POU2F3 in Human Melanoma. POU2F3 was detected in immersion fixed paraffin-embedded sections of human melanoma using Mouse Anti-Human POU2F3 Monoclonal Antibody (Catalog # MAB11625) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: POU2F3

POU2F3 is a 47 kDa transcription factor involved in the regulation of the differentiation of keratinocytes. It is also a part of the POU2F3-POU2AF2/POU2AF3 complex that drives the expression of tuft-cell-specific genes. Normal and neoplastic tuft cells share a genetic requirement for POU2F3. POU2F3 is utilized as an ancillary marker for the diagnosis of neuroendocrine-low small cell lung cancer. It is also positive in mesenchymal and neuroectodermal tumors including synovial carcinoma, solitary fibrous tumor, glioblastoma, Wilms tumor and melanoma.

References

Hildesheim J, Kühn U, Yee CL, Foster RA, Yancey KB, Vogel JC. The hSkn-1a POU transcription factor enhances epidermal stratification by promoting keratinocyte proliferation. J Cell Sci. 2001 May;114(Pt 10):1913-23. doi: 10.1242/jcs.114.10.1913. PMID: 11329378.
Wu XS, He XY, Ipsaro JJ, Huang YH, Preall JB, Ng D, Shue YT, Sage J, Egeblad M, Joshua-Tor L, Vakoc CR. OCA-T1 and OCA-T2 are coactivators of POU2F3 in the tuft cell lineage. Nature. 2022 Jul;607(7917):169-175. doi: 10.1038/s41586-022-04842-7. Epub 2022 May 16. PMID: 35576971; PMCID: PMC9419707.
Kaczorowski M, Ylaya K, Chłopek M, Lasota J, Miettinen M. Expression of POU2F3 Transcription Factor and POU2AF2, POU2F3 Coactivator, in Tuft Cell-like Carcinoma and Other Tumors. Am J Surg Pathol. 2024 Sep 25. doi: 10.1097/PAS.0000000000002313. Epub ahead of print. PMID: 39319626.

Long Name

POU class 2 homeobox 3

Entrez Gene IDs

25833 (Human); 18988 (Mouse); 116544 (Rat)

Alternate Names

Epoc-1; MGC126698; OCT11; OCT-11; OCT11FLJ40063; Octamer-binding protein 11; Octamer-binding transcription factor 11; OTF11; OTF-11; PLA1; PLA-1; POU class 2 homeobox 3; POU domain class 2, transcription factor 3; POU domain, class 2, transcription factor 3; POU transcription factor; POU2F3; Skn-1a; Transcription factor PLA-1; Transcription factor Skn-1