Human POU3F2 Antibody

Catalog #: MAB7427 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7427
MAB7427-SP

Detection of Human POU3F2 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human POU3F2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human POU3F2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) POU5F1 or rhPOU4F3 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 761340

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human POU3F2
Asn7-His59
Accession # P20265

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human POU3F2 by Western Blot. Western blot shows lysates of A375 human melanoma cell line, Bowes human melanoma cell line, and SK-Mel-28 human malignant melanoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human POU3F2 Monoclonal Antibody (Catalog # MAB7427) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for POU3F2 at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: POU3F2

POU3F2, also called Brn2, is a 47 kDa (predicted) member of the Octamer-binding transcription factor family. It is mainly expressed in the brain, especially in neuroectodermal cell lineages. With transcription factors ASCL1/Mash1 and Myt1L, POU3F2 has been used to induce neuronal differentiation of human stem cells and mouse fibroblasts. The full length transcript of POU3F2 produces the transcription factor N-Oct3, while alternate transcription initiation at amino acids 181 and 200 produce N-Oct5A and N-Oct5B isoforms, respectively. The sequence used as an immunogen is present only in N-Oct3 and is identical in human and mouse, while rat POU3F2 shows one amino acid difference.

Long Name

POU Class 3 Homeobox 2

Entrez Gene IDs

5454 (Human)

Alternate Names

Brain-2; Brain-specific homeobox/POU domain protein 2; BRN2; brn-2; BRN2brain-2; Nervous system-specific octamer-binding transcription factor N-Oct-3; Oct7; oct-7; OCT7POU domain class 3, transcription factor 2; Octamer-binding protein 7; Octamer-binding transcription factor 7; OTF7; OTF-7; POU class 3 homeobox 2; POU domain, class 3, transcription factor 2; POU3F2; POUF3