Human PP1 Inhibitor-2 Antibody

Catalog # Availability Size / Price Qty
MAB4719
MAB4719-SP
Detection of Human PP1 Inhibitor‑2 by Western Blot.
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Human PP1 Inhibitor-2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human PP1 Inhibitor‑2 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2B Clone # 488123
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human PP1 Inhibitor‑2
Met1-Ser205
Accession # P41236
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human PP1 Inhibitor‑2 antibody by Western Blot. View Larger

Detection of Human PP1 Inhibitor‑2 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human PP1 Inhibitor-2 Monoclonal Antibody (Catalog # MAB4719) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human PP1 Inhibitor-2 (5 ng) was included. A specific band for PP1 Inhibitor-2 was detected at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PP1 Inhibitor-2

Protein Phosphatase 1 Inhibitor-2 (PP1 Inhibitor-2), also called PP1 regulatory subunit 2 (PP1R2), I2, and IPP2, binds to PP1 through an RVxF consensus sequence. The complex inactivates PP1, preventing it from dephosphorylating substrates such as glycogen phosphorylase. Unlike PP1 Inhibitor-1, the binding of PP1 Inhibitor-2 is Protein Kinase A phosphorylation-independent. Dissociation of the PP1 Inhibitor-2 complex is enhanced by phosphorylation at T72 by GSK-3, ERK, or CDK. Overexpression of PP1 Inhibitor-2 in mouse heart improves cardiac function without changing organ size.

Long Name
Protein Phosphatase 1 Inhibitor 2
Entrez Gene IDs
5504 (Human); 66849 (Mouse); 192361 (Rat)
Alternate Names
IPP2; IPP-2; IPP2phosphatase inhibitor 2; MGC87148; phosphoprotein phosphatase; PP1 Inhibitor2; PP1 Inhibitor-2; PPP1R2; protein phosphatase 1, regulatory (inhibitor) subunit 2; protein phosphatase inhibitor 2

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