Home / Pref-1/DLK1/FA1 / Human Pref-1/DLK1/FA1 Alexa Fluor® 488-conjugated Antibody

Human Pref-1/DLK1/FA1 Alexa Fluor® 488-conjugated Antibody

Catalog #: FAB1144G
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB1144G-100
FAB1144G-025
Detection of Pref‑1/DLK1/FA1 in HepG2 Human Cell Line by Flow Cytometry.
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Citations (2)
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Human Pref-1/DLK1/FA1 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Pref‑1/DLK1/FA1 in direct ELISAs and Western blots. Shows approximately 50% cross-reactivity with recombinant mouse Pref‑1/DLK1/FA1.
Source
Monoclonal Mouse IgG2B Clone # 211309
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Pref-1/DLK1/FA1 long isoform
Ala24-Pro297 (with Arg248Pro and Lys295Ser substitutions)
Accession # P80370
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of Pref‑1/DLK1/FA1 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Pref-1/DLK1/FA1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB1144G, filled histogram) or isotype control antibody (IC0041G, open histogram). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Pref-1/DLK1/FA1

Pref-1 (Preadipocyte Factor 1), also known as DLK1 and FA1, is a 58-65 kDa member of the Notch/Serrata/Delta family of proteins.  It is expressed in prechondrocytes and preadipocytes and appears to block progenitor cell differentiation into mature cell lineages.  Mature human Pref-1 is a 360 amino acid (aa) type I transmembrane N- and O-linked glycoprotein.  It contains a 280 aa extracellular region (aa 24-303), a 24 aa transmembrane segment (aa 304-327), and a 56 aa cytoplasmic domain (aa 328-383).  The extracellular region contains six EGF-like domains, and undergoes proteolytic cleavage to generate a bioactive 50 kDa fragment, plus three 25-31 kDa fragments that show no activity.  There are multiple potential splice variants.  One shows a deletion of aa 229-301, a second possesses a six aa substitution for aa 1-52, a third shows a deletion of aa 210-277, while a fourth contains a six aa substitution for aa 207-383.  Over aa 24-297, human Pref-1 shares 82% aa identity with mouse Pref-1.

Long Name
Preadipocyte Factor-1/Protein delta Homolog 1/Fetal Antigen 1
Entrez Gene IDs
8788 (Human); 13386 (Mouse)
Alternate Names
delta-like 1 homolog (Drosophila); DLK; DLK1; DLK-1; FA1; FA1fetal antigen 1; pG2; pG2delta-like homolog (Drosophila); preadipocyte factor 1; Pref1; Pref-1; protein delta homolog 1; secredeltin; ZOG

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Citations for Human Pref-1/DLK1/FA1 Alexa Fluor® 488-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Dlk1 is a novel adrenocortical stem/progenitor cell marker that predicts malignancy in adrenocortical carcinoma
    Authors: Mariniello, K;Pittaway, JFH;Altieri, B;Borges, KS;Hadjidemetriou, I;Ribeiro, C;Ruiz-Babot, G;Lim, JA;Foster, J;Cleaver, J;Sosabowski, J;Rahman, N;Doroszko, M;Hantel, C;Sigala, S;Abate, A;Tamburello, M;Kiseljak-Vassiliades, K;Wierman, M;Parvanta, L;Abdel-Aziz, TE;Chung, TT;Marco, AD;Palazzo, F;Gomez-Sanchez, CE;Taylor, DR;Rayner, O;Ronchi, CL;Gaston-Massuet, C;Sbiera, S;Drake, WM;Rognoni, E;Kroiss, M;Breault, DT;Fassnacht, M;Guasti, L;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Whole Cells
    Applications: FACS
  2. Single-cell analysis reveals fibroblast clusters linked to immunotherapy resistance in cancer
    Authors: Y Kieffer, HR Hocine, G Gentric, F Pelon, C Bernard, B Bourachot, S Lameiras, L Albergante, C Bonneau, A Guyard, K Tarte, A Zinovyev, S Baulande, G Zalcman, A Vincent-Sa, F Mechta-Gri
    Cancer Discov, 2020-05-20;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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