Human Pref-1/DLK1/FA1 Antibody Summary
Ala24-Pro297 (with Arg248Pro and Lys295Ser substitutions)
Accession # P80370
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Pref‑1/DLK1/FA1 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Pref-1/DLK1/FA1 Monoclonal Antibody (Catalog # MAB1144, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
View Larger
Pref‑1/DLK1/FA1 in HepG2 Human Cell Line. Pref-1/DLK1/FA1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Pref-1/DLK1/FA1 Monoclonal Antibody (Catalog # MAB1144) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Pref-1/DLK1/FA1
Pref-1 (Preadipocyte factor 1; also DLK-1 and FA1) is a 58-65 kDa member of the Notch/Serrata/Delta family of proteins. It is expressed in prechondrocytes and preadipocytes and appears to block progenitor cell differentiation into mature cell lineages. Mature human Pref-1 is a 360 amino acid (aa) type I transmembrane N- and O-linked glycoprotein. It contains a 280 aa extracellular region (aa 24-303), a 24 aa transmembrane segment (aa 304-327), and a 56 aa cytoplasmic domain (aa 328-383). The extracellular region contains six EGF-like domains, and undergoes proteolytic cleavage to generate a bioactive 50 kDa fragment, plus three 25‑31 kDa fragments that show no activity. There are multiple potential splice variants. One shows a deletion of aa 229-301, a second possesses a six aa substitution for aa 1‑52, a third shows a deletion of aa 210-277, while a fourth contains a six aa substitution for aa 207-383. Over aa 24-297, human Pref-1 shares 82% aa identity with mouse Pref-1.
Product Datasheets
Citations for Human Pref-1/DLK1/FA1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
6
Citations: Showing 1 - 6
Filter your results:
Filter by:
-
Obesity induces preadipocyte CD36 expression promoting inflammation via the disruption of lysosomal calcium homeostasis and lysosome function
Authors: X Luo, Y Li, P Yang, Y Chen, L Wei, T Yu, J Xia, XZ Ruan, L Zhao, Y Chen
EBioMedicine, 2020-06-06;56(0):102797.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Effects of Delta-Like Noncanonical Notch Ligand 1 Expression of Human Fetal Liver Hepatoblasts on Hematopoietic Progenitors
Authors: JC Gerlach, RL Thompson, B Gridelli, E Schmelzer
Stem Cells Int, 2019-03-18;2019(0):7916275.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Interaction of delta-like 1 homolog (Drosophila) with prohibitins and its impact on tumor cell clonogenicity.
Authors: Begum A, Lin Q, Yu C, Kim Y, Yun Z
Mol Cancer Res, 2013-11-18;12(1):155-64.
Species: Human
Sample Types: Cell Lysates
Applications: ICC, Immunoprecipitation -
Hypoxia-regulated delta-like 1 homologue enhances cancer cell stemness and tumorigenicity.
Authors: Kim Y, Lin Q, Zelterman D, Yun Z
Cancer Res., 2009-12-15;69(24):9271-80.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Preadipocytes mediate lipopolysaccharide-induced inflammation and insulin resistance in primary cultures of newly differentiated human adipocytes.
Authors: Chung S, Lapoint K, Martinez K, Kennedy A, Boysen Sandberg M, McIntosh MK
Endocrinology, 2006-07-27;147(11):5340-51.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Reck enables cerebrovascular development by promoting canonical Wnt signaling
Authors: Ulrich F, Carretero-Ortega J, MenEndez J et al.
Development.
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human Pref-1/DLK1/FA1 Antibody
Average Rating: 4.7 (Based on 3 Reviews)
Have you used Human Pref-1/DLK1/FA1 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
