Human Presenilin-1 N-Terminal Fragment Antibody

Catalog #: MAB149
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB149
MAB149-SP

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Presenilin-1 N-Terminal Fragment Antibody Summary

Species Reactivity

Human

Specificity

Detects human Presenilin‑1 N-Terminal Fragment in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) Presenilin‑2 N-Terminal Fragment, rhPresenilin‑1 C-Terminal Fragment, or rhPresenilin‑2 C-Terminal Fragment.

Source

Monoclonal Mouse IgG_2B Clone # 121121

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Presenilin‑1 N-Terminal Fragment
Lys1-Lys80
Accession # P49768

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Presenilin‑1 N-Terminal Fragment

Immunohistochemistry

1-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Presenilin‑1 in Human Brain. Presenilin-1 was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Mouse Anti-Human Presenilin-1 N-Terminal Fragment Monoclonal Antibody (Catalog # MAB149) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Presenilin-1

Presenilin‑1 (PS-1) is a multipass lysosomal membrane protein that is a component of the gamma secretase complex. It is cleaved by endoproteolysis to generate
N‑ and C‑terminal fragments. Some mutations in PS-1 are associated with the risk of early onset familial Alzheimer's disease.

Entrez Gene IDs

5663 (Human)

Alternate Names

AD3EC 3.4.23.-; Alzheimer disease 3; EC 3.4.23; FAD; presenilin 1; Presenilin1; Presenilin-1; Protein S182; PS-1; PS1presenilin-1; PSEN1; PSNL1; S182

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human Presenilin-1 N-Terminal Fragment Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Differential IL-23 requirement for IL-22 and IL-17A production during innate immunity against Salmonella enterica serovar Enteritidis.
Authors: Siegemund S, Schutze N, Schulz S, Wolk K, Nasilowska K, Straubinger RK, Sabat R, Alber G
Int. Immunol., 2009-03-18;21(5):555-65.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development