Human Presenilin-2 N-Terminal Fragment Antibody

Catalog #: AF153 Datasheet / COA / SDS

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AF153
AF153-SP

All Presenilin-2 Antibodies

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Human Presenilin-2 N-Terminal Fragment Antibody Summary

Species Reactivity

Human

Specificity

Detects human Presenilin‑2 N-Terminal Fragment in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human Presenilin‑1 N-Terminal Fragment (aa 1‑80) and recombinant human Presenilin‑2 C-Terminal Fragment (aa 308‑388) is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Presenilin-2
Leu2-Thr80
Accession # P49810

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Presenilin‑2 N-Terminal Fragment

Immunohistochemistry

5-15 µg/mL

Immersion fixed paraffin-embedded sections of human brain (hippocampus)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Presenilin-2

Presenilin‑2 (PS-2 and PSEN2) is a catalytic subunit of the gamma-secretase complex. Presenilin‑2 may play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. Heterogeneous proteolytic processing of Presenilin‑2 generates N-terminal (aa 1-297) and C-terminal (aa 298-248) fragments. Presenilin 1 and Presenilin 2 determine gamma-secretase activity, which is responsible for cleaving the amyloid precursor protein (APP). In turn, APP cleavage can produce amyloid-beta, which is the primary component of plaques in the brains of Alzheimer’s disease patients. Full length, approximately 50 kDa, and the approximately 30 kDa N-terminal fragment of Presenilin-2 were identified by western blotting in extracted rodent pancreas but only the 30 kDa fragment was detected in mouse islets and human insulinoma. Within a sequence correspondent to the immunogen, human PS-2 shares 87% aa sequence identity with mouse and rat PS-2.

Entrez Gene IDs

5664 (Human)

Alternate Names

AD3LP; AD4AD3L; AD5; E5-1; EC 3.4.23; EC 3.4.23.-; presenilin 2 (Alzheimer disease 4); Presenilin2; Presenilin-2; PS-2; PS2CMD1V; PSEN2; PSNL2; STM2STM-2