Home / Angiopoietin-like Protein 4/ANGPTL4 / Human/Primate Angiopoietin-like Protein 4/ANGPTL4 Biotinylated Antibody

Human/Primate Angiopoietin-like Protein 4/ANGPTL4 Biotinylated Antibody

Catalog #: BAF3485
7 Citations Datasheet / COA / SDS
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BAF3485
Product Details
Citations (7)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Primate Angiopoietin-like Protein 4/ANGPTL4 Biotinylated Antibody Summary

Species Reactivity
Human, Primate
Specificity
Detects human ANGPTL4 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human (rh) ANGPTL3, rhAngiopoietin-1, -2, -3, and -4 is observed.In Western blots, approximately 1% cross-reactivity with rhANGPLT4 N-terminal Fragment is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Angiopoietin-like 4
Leu165-Ser406
Accession # Q9BY76
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Angiopoietin‑like 4/ANGPTL4 (Catalog # 4487-AN)

Human/Primate Angiopoietin-like Protein 4/ANGPTL4 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human/Primate Angiopoietin‑like Protein 4/ANGPTL4 Antibody (Catalog # AF3485)

Standard: Recombinant Human Angiopoietin-like 4 Protein, CF (Catalog # 4487-AN)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Angiopoietin-like Protein 4/ANGPTL4

ANGPTL4, also known as PPAR gamma angiopoietin-related protein (PGAR), hepatic fibrinogen/angiopoietin-related protein (HFARP) and fasting-induced adipose factor (FIAF), is a secreted protein that shares structural homology with angiopoietins. It contains an N-terminal coiled-coil region that mediates covalent homo-oligomerization and a C-terminal fibrinogen-like domain. ANGPTL4 undergoes proteolytic processing and releases the C-terminal domain, which circulates as a monomer. At least 2 additional splice isoforms exist. ANGPTL4 is most highly expressed in adipose tissues. Its expression is up-regulated in endothelial cells and cardiomyocytes during hypoxia. ANGPTL4 is involved in the regulation of lipid and glucose metabolism. It has also been associated with angiogenesis. The amino acid sequence of human ANGPTL4 is 81%, 76% and 81% identical to that of porcine, mouse and canine ANGPTL4, respectively.

Entrez Gene IDs
51129 (Human); 57875 (Mouse); 362850 (Rat)
Alternate Names
Angiopoietin like Protein 4; angiopoietin-like 4; Angiopoietin-like Protein 4; ANGPTL4; ARP4fasting-induced adipose factor; FIAF; FIAFhepatic angiopoietin-related protein; Hepatic fibrinogen/angiopoietin-related protein; HFARP; HFARPANGPTL2; NL2; peroxisome proliferator-activated receptor (PPAR) gamma inducedangiopoietin-related protein; PGAR; PGARangiopoietin-related protein 4; pp1158; PPARG angiopoietin related protein

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Citations for Human/Primate Angiopoietin-like Protein 4/ANGPTL4 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Effect of diet-induced weight loss on angiopoietin-like protein 4 and adipose tissue lipid metabolism in overweight and obese humans
    Authors: BW van der Ko, RG Vink, JWE Jocken, NJT Roumans, GH Goossens, ECM Mariman, MA van Baak, EE Blaak
    Physiol Rep, 2018-07-01;6(13):e13735.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development (Detection)
  2. Plasma angiopoietin-like 4 is related to phospholipid transfer protein activity in diabetic and non-diabetic subjects: role of enhanced low grade inflammation
    Authors: EG Gruppen, S Kersten, RPF Dullaart
    Lipids Health Dis, 2018-03-27;17(1):60.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development (Detection)
  3. Regulation of angiopoietin-like 4 and lipoprotein lipase in human adipose tissue
    Authors: W Dijk, S Schutte, EO Aarts, IMC Janssen, L Afman, S Kersten
    J Clin Lipidol, 2018-02-17;0(0):.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Detection
  4. ANGPTL4 mediates shuttling of lipid fuel to brown adipose tissue during sustained cold exposure
    Authors: Wieneke Dijk, Markus Heine, Laurent Vergnes, Mariëtte R Boon, Gert Schaart, Matthijs K C Hesselink et al.
    eLife
  5. Inflammation increases plasma angiopoietin-like protein 4 in patients with the metabolic syndrome and type 2 diabetes
    Authors: Nathanja Tjeerdema, Anastasia Georgiadi, Jacqueline T Jonker, Marjolijn van Glabbeek, Reza Alizadeh Alizadeh Dehnavi, Jouke T Tamsma et al.
    BMJ Open Diabetes Research & Care
  6. Short-chain fatty acids stimulate angiopoietin-like 4 synthesis in human colon adenocarcinoma cells by activating peroxisome proliferator-activated receptor gamma.
    Authors: Alex S, Lange K, Amolo T, Grinstead J, Haakonsson A, Szalowska E, Koppen A, Mudde K, Haenen D, Al-Lahham S, Roelofsen H, Houtman R, van der Burg B, Mandrup S, Bonvin A, Kalkhoven E, Muller M, Hooiveld G, Kersten S
    Mol Cell Biol, 2013-01-22;33(7):1303-16.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  7. Caloric restriction and exercise increase plasma ANGPTL4 levels in humans via elevated free fatty acids.
    Authors: Kersten S, Lichtenstein L, Steenbergen E, Mudde K, Hendriks HF, Hesselink MK, Schrauwen P, Muller M
    Arterioscler. Thromb. Vasc. Biol., 2009-04-02;29(6):969-74.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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