Human/Primate CXCL16 Antibody Summary
Asn49-Pro137
Accession # NP_071342
Applications
Human/Primate CXCL16 Sandwich Immunoassay
0.05-0.25 μg/mL in the presence of 20 ng/mL Recombinant Human CXCL16 Chemokine Domain.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Chemotaxis Induced by CXCL16 and Neutralization by Human CXCL16 Antibody. Recombinant Human CXCL16 Chemokine Domain (Catalog # 976-CX) induces chemotaxis in BaF3 mouse pro-B cell line transfected with mouse CXCR6 in a dose-dependent manner (orange line). Chemotaxis elicited by Recombinant Human CXCL16 Chemokine Domain (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Primate CXCL16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF976). The ND50 is typically 0.05-0.25 µg/mL.
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CXCL16 in Human Lymph Node. CXCL16 was detected in immersion fixed paraffin-embedded sections of human lymph node using Goat Anti-Human/Primate CXCL16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF976) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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CXCL16 in Human Lymphoma. CXCL16 was detected in immersion fixed paraffin-embedded sections of human lymphoma using Goat Anti-Human/Primate CXCL16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF976) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL16
CXC chemokine ligand 16 (CXCL16) is a type I membrane protein containing a non-ELR motif-containing CXC chemokine domain in its extracellular region. Together with Fractalkine (CX3CL1), CXCL16 constitute the only two transmembrane chemokines within the superfamily. The gene for human CXCL16 predicts a 273 amino acid (aa) residue precursor protein with a putative signal peptide, a CXC chemokine domain, a mucin-like spacer region, a transmembrane domain and a cytoplasmic domain with a potential tyrosine phosphorylation and SH2 protein-binding site. Mouse and human CXCL16 share 70% aa sequence similarity within their chemokine domains and 49% overall aa sequence identity. By northern blot analysis, CXCL16 expression is detected in various human organs except for brain, bone marrow, skeletal muscle or colon. By flow cytometry, CXCL16 has been detected on the surface CD19+ B cells, CD14+ monocytes/macrophages, and CD11c+ splenic and lymph node dendritic cells. Functional CXCL16 can be shed from the cell surface as an approximately 35 kDa soluble protein. The functional receptor for CXCL16 has been identified as CXCR6 (also known as Bonzo, STRL33 or TYMSTR), a receptor previously shown to be a co-receptor for HIV entry. CXCL16 has also been independently cloned and named SRPSOX (scavenger receptor that binds phosphatidylserine and oxidized lipoprotein). It was shown to be a specific receptor for OxLDL but not LDL or acetyl-LDL.
- Matloubian, M. et al. (2000) Nature Immun. 1:298.
- Shimaoka, T. et al. (2000) J. Biol. Chem. 275:40663.
- Wilbanks, A. et al. (2001) J. Immun. 166:5145.
Product Datasheets
Citations for Human/Primate CXCL16 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
16
Citations: Showing 1 - 10
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Evidence that CXCL16 is a potent mediator of angiogenesis and is involved in endothelial progenitor cell chemotaxis : studies in mice with K/BxN serum-induced arthritis
Authors: Takeo Isozaki, Ali S. Arbab, Christian S. Haas, M. Asif Amin, Monica D. Arendt, Alisa E. Koch et al.
Arthritis & Rheumatism
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Expression and function of CXCL16 in a novel model of gout
Authors: Jeffrey H. Ruth, Monica D. Arendt, M. Asif Amin, Salahuddin Ahmed, Hubert Marotte, Bradley J. Rabquer et al.
Arthritis & Rheumatism
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Elevated serum CXCL16 is an independent predictor of poor survival in ovarian cancer and may reflect pro-metastatic ADAM protease activity
Authors: M J M Gooden, V R Wiersma, A Boerma, N Leffers, H M Boezen, K A ten Hoor et al.
British Journal of Cancer
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Disruption of cholesterol homeostasis triggers periodontal inflammation and alveolar bone loss
Authors: Tran, TT;Lee, G;Huh, YH;Chung, KH;Lee, SY;Park, KH;Kwon, SH;Kook, MS;Chun, JS;Koh, JT;Ryu, JH;
Experimental & molecular medicine
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Chemokine CXCL16 mediates acinar cell necrosis in cerulein induced acute pancreatitis in mice
Authors: Y Sakuma, Y Kodama, T Eguchi, N Uza, Y Tsuji, M Shiokawa, T Maruno, K Kuriyama, Y Nishikawa, Y Yamauchi, M Tsuda, T Ueda, T Matsumori, T Morita, T Tomono, N Kakiuchi, A Mima, Y Sogabe, S Marui, T Kuwada, A Okada, T Watanabe, H Nakase, T Chiba, H Seno
Sci Rep, 2018-06-11;8(1):8829.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Long Noncoding RNAs and mRNA Regulation in Peripheral Blood Mononuclear Cells of Patients with Chronic Obstructive Pulmonary Disease
Authors: X Qu, X Dang, W Wang, Y Li, D Xu, D Shang, Y Chang
Mediators Inflamm., 2018-03-13;2018(0):7501851.
Species: Human
Sample Types: Cell Culture Supernates, Protein
Applications: Neutralization -
CXCL16 and CXCR6 are coexpressed in human lung cancer in vivo and mediate the invasion of lung cancer cell lines in vitro.
Authors: Hu, Weidong, Liu, Yue, Zhou, Wenhui, Si, Lianlian, Ren, Liang
PLoS ONE, 2014-06-04;9(6):e99056.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: Flow Cytometry, ICC, IHC-P -
Scavenger receptors in human airway epithelial cells: role in response to double-stranded RNA.
Authors: Dieudonne A, Torres D, Blanchard S, Taront S, Jeannin P, Delneste Y, Pichavant M, Trottein F, Gosset P
PLoS ONE, 2012-08-07;7(8):e41952.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
CXCR6-CXCL16 interaction in the pathogenesis of Juvenile Idiopathic Arthritis.
Authors: Martini G, Cabrelle A, Calabrese F, Carraro S, Scquizzato E, Teramo A, Facco M, Zulian F, Agostini C
Clin. Immunol., 2008-08-28;129(2):268-76.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Expression and potential function of the CXC chemokine CXCL16 in pancreatic ductal adenocarcinoma.
Authors: Wente MN, Gaida MM, Mayer C, Michalski CW, Haag N, Giese T, Felix K, Bergmann F, Giese NA, Friess H
Int. J. Oncol., 2008-08-01;33(2):297-308.
Species: Human
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC-P, Western Blot -
Overexpression of CXCL16 and its receptor CXCR6/Bonzo promotes growth of human schwannomas.
Authors: Held-Feindt J, Rehmke B, Mentlein R, Hattermann K, Knerlich F, Hugo HH, Ludwig A, Mehdorn HM
Glia, 2008-05-01;56(7):764-74.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: IHC, Neutralization -
Inhibition of angiogenesis by interleukin-4 gene therapy in rat adjuvant-induced arthritis.
Authors: Haas CS, Amin MA, Allen BB, Ruth JH, Haines GK, Woods JM, Koch AE
Arthritis Rheum., 2006-08-01;54(8):2402-14.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
CXCL16-mediated cell recruitment to rheumatoid arthritis synovial tissue and murine lymph nodes is dependent upon the MAPK pathway.
Authors: Ruth JH, Haas CS, Park CC, Amin MA, Martinez RJ, Haines GK, Shahrara S, Campbell PL, Koch AE
Arthritis Rheum., 2006-03-01;54(3):765-78.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
EPHB4 regulates chemokine-evoked trophoblast responses: a mechanism for incorporating the human placenta into the maternal circulation.
Authors: Red-Horse K, Kapidzic M, Zhou Y, Feng KT, Singh H, Fisher SJ
Development, 2005-08-17;132(18):4097-106.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Evaluation of CXCL10 as a novel serum marker for predicting liver metastasis and prognosis in colorectal cancer
Authors: Yuji Toiyama, Hiroyuki Fujikawa, Mikio Kawamura, Kohei Matsushita, Susumu Saigusa, Koji Tanaka et al.
International Journal of Oncology
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The perioperative time course and clinical significance of the chemokine CXCL 16 in patients undergoing cardiac surgery
Authors: Daniela Dreymueller, Andreas Goetzenich, Christoph Emontzpohl, Josefin Soppert, Andreas Ludwig, Christian Stoppe
Journal of Cellular and Molecular Medicine
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