Human/Primate CXCL16 Biotinylated Antibody

Catalog #: BAF976 Datasheet / COA / SDS

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BAF976

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Human/Primate CXCL16 Biotinylated Antibody Summary

Species Reactivity

Human, Primate

Specificity

Detects human CXCL16 in ELISAs and Western blots. In Sandwich ELISAs, less than 0.05% cross-reactivity with recombinant mouse (rm) CXCL16, rm6Ckine, recombinant human (rh) BLC, rhCTACK, rhIL-8, rhPF4, and rhLymphotactin is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human CXCL16
Asn49-Pro137
Accession # NP_071342

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human CXCL16 Chemokine Domain (Catalog # 976-CX)

Flow Cytometry

0.25 µg/10⁶ cells

Human monocyte-derived dendritic cells

Immunohistochemistry

5-15 µg/mL

See below

Human/Primate CXCL16 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Human/Primate CXCL16 Antibody (Catalog # AF976)

Standard: Recombinant Human CXCL16 (Histidine-tagged) Protein (Catalog # 1164-CX)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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CXCL16 in Human Lymphoma Tissue. CXCL16 was detected in immersion fixed paraffin-embedded sections of human lymphoma tissue using Goat Anti-Human/Primate CXCL16 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF976) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL16

CXC chemokine ligand 16 (CXCL16) is a type I membrane protein containing a non-ELR motif-containing CXC chemokine domain in its extracellular region. Together with Fractalkine (CX3CL1), CXCL16 constitute the only two transmembrane chemokines within the superfamily. The gene for human CXCL16 predicts a 273 amino acid (aa) residue precursor protein with a putative signal peptide, a CXC chemokine domain, a mucin-like spacer region, a transmembrane domain and a cytoplasmic domain with a potential tyrosine phosphorylation and SH2 protein-binding site. Mouse and human CXCL16 share 70% aa sequence similarity within their chemokine domains and 49% overall aa sequence identity. By northern blot analysis, CXCL16 expression is detected in various human organs except for brain, bone marrow, skeletal muscle or colon. By flow cytometry, CXCL16 has been detected on the surface CD19⁺ B cells, CD14⁺ monocytes/macrophages, and CD11c⁺ splenic and lymph node dendritic cells. Functional CXCL16 can be shed from the cell surface as an approximately 35 kDa soluble protein. The functional receptor for CXCL16 has been identified as CXCR6 (also known as Bonzo, STRL33 or TYMSTR), a receptor previously shown to be a co-receptor for HIV entry. CXCL16 has also been independently cloned and named SRPSOX (scavenger receptor that binds phosphatidylserine and oxidized lipoprotein). It was shown to be a specific receptor for OxLDL but not LDL or acetyl-LDL.

References

Matloubian, M. et al. (2000) Nature Immun. 1:298.
Shimaoka, T. et al. (2000) J. Biol. Chem. 275:40663.
Wilbanks, A. et al. (2001) J. Immun. 166:5145.

Entrez Gene IDs

58191 (Human); 66102 (Mouse)

Alternate Names

chemokine (C-X-C motif) ligand 16; CXC chemokine ligand 16; CXCL16; CXCLG16; Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein; SCYB16; Small-inducible cytokine B16; SRPSOXC-X-C motif chemokine 16; SR-PSOXTransmembrane chemokine CXCL16