Human/Primate IL-17F Biotinylated Antibody

Name: Human/Primate IL-17F Biotinylated Antibody
Brand: R&D Systems
SKU: BAF1335
Rating: 5 (1 reviews)

Catalog #: BAF1335 1 Review Datasheet / COA / SDS

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BAF1335

All IL-17F Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Primate IL-17F Biotinylated Antibody Summary

Species Reactivity

Human, Primate

Specificity

Detects human IL-17F in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross‑reactivity with rhIL-17, rhIL-17B, rhIL-17C, rhIL‑17D, rhIL‑17E, and rmIL‑17F is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human IL-17F (R&D Systems, Catalog # 1335-IL)
Arg31-Gln163
Accession # AAK83350

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human IL‑17F (Catalog # 1335-IL)

Human/Primate IL-17F Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Human/Primate IL‑17F Antibody (Catalog # AF1335)

Standard: Recombinant Human IL-17F Protein (Catalog # 1335-IL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17F

The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17A through IL-17F), are secreted, structurally related proteins that share a conserved cystine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus. With the exception of IL-17B, which exists as a non-covalently linked dimer, all IL-17 family members are disulfide-linked dimers. IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2).

Human IL-17F cDNA encodes a 163 aa protein with a putative 30 aa signal peptide. Among IL-17 family members, IL-17F is most closely related to IL-17A (approximately 44% aa sequence homology), but shares only limited sequence homology (16 - 30%) with IL-17B, C, D and E. Human and mouse IL-17F share 55% sequence identity. IL-17F is expressed in activated CD4+ T-cells and activated monocytes. Five receptors (IL-17 RA, B, C, D and E) have been identified (5). Although the ligands for IL-17 RD and E are not known yet, it is reported that IL-17 RA binds IL-17A, and IL-17 RB binds IL-17B and IL-17E. IL-17 RC binds IL-17A and IL-17F with similarly high affinity and functions as a receptor for both IL-17A and IL-17F (5, 6). The biological activities mediated by IL-17F are similar to those of IL-17. IL-17F stimulates production of IL-6, IL-8, G-CSF, and regulates cartilage matrix turnover by increasing matrix release and inhibiting new matrix synthesis (4). IL-17F also inhibits angiogenesis and induces production of IL-2, TGF-beta, and monocyte chemoattractant protein-1 in endothelial cells (3).

References