Human Pro BDNF Antibody

Catalog #: MAB3175 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3175
MAB3175-SP

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Human Pro BDNF Antibody Summary

Species Reactivity

Human

Specificity

Detects human Pro-BDNF in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) BDNF (mature), rh beta -NGF, rhNT-3, rhNT-4, rhPro-NT-3 or rhPro-NT-4 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 584421

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Pro-BDNF
Ala19-Arg247
Accession # P23560

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

Immersion fixed paraffin-embedded sections of hippocampus

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Detection of BDNF in Hippocampus. BDNF was detected in immersion fixed paraffin-embedded sections of hippocampus using Mouse Anti-Human Pro BDNF Monoclonal Antibody (Catalog # mab3175) at 15 µg/ml overnight at 4 degrees Celsius followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BDNF

BDNF is a member of the NGF family of neurotrophic factors that are required for the differentiation and survival of neuronal subpopulations in the central and peripheral nervous systems. BDNF functions through interactions with the TrkB receptor tyrosine kinase and the low affinity neurotrophin receptor, p75 (NTR). The human BDNF cDNA encodes 247 amino acids (aa). Cleavage of an 18 aa signal sequence produces an approximately 35 kDa Pro-BDNF form. The N-terminal pro region of BDNF is removed by tPA and furin to release biologically active, 14 kDa BDNF. The propeptides of human and mouse BDNF share 93% aa sequence identity.

Long Name

Brain-derived Neurotrophic Factor

Entrez Gene IDs

627 (Human); 12064 (Mouse); 24225 (Rat)

Alternate Names

Abrineurin; ANON2; BDNF; brain-derived neurotrophic factor; BULN2; MGC34632; Neurotrophin