Human Pro-Corticoliberin/Pro-CRH Antibody Summary
Leu25-Lys196
Accession # P06850
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Pro-Corticoliberin/Pro-CRH by Western Blot. Western blot shows lysates of human cerebellum and hypothalamus tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Pro-Corticoliberin/Pro-CRH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5805) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Pro-Corticoliberin/Pro-CRH at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Pro-Corticoliberin/Pro-CRH
Pro-Corticoliberin (also Pro-CRF and Pro-CRH) is a predicted 19 kDa member of the CRF/Urotensin I family of hormones. It is expressed by placental synctiotrophoblasts, zona fasciculata adrenal cells and paraventricular neurons, and is induced by glucocorticoids. Pro‑Corticoliberin is the precursor to a mature, 41 amino acid (aa) amidated Corticoliberin that induces pituitary ACTH and subsequent cortisol production by the adrenal. Human Pro‑Corticoliberin is 170 aa in length (aa 25‑194). If unprocessed, it undergoes N-terminal amidation to generate a polypeptide 168 aa in length (aa 27‑194). Secreted Pro‑Corticoliberin is both bioactive and binds to 37 kDa Corticoliberin-PB. In the placenta, Pro‑Corticoliberin is only occasionally cleaved to generate mature Corticoliberin (aa 152‑192); in the brain, it undergoes active internal proteolysis to generate multiple fragments, including the 5 kDa mature peptide. Over aa 25‑196, human Pro‑Corticoliberin shares 78% aa identity with mouse Pro‑Corticoliberin.
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